Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell
DISEASE(S): Disease Free
SUBMITTER: Christian Behrends
LAB HEAD: Christian Behrends
PROVIDER: PXD052316 | Pride | 2024-10-17
REPOSITORIES: Pride
Action | DRS | |||
---|---|---|---|---|
20230614_SL_345.raw | Raw | |||
20230614_SL_346.raw | Raw | |||
20230614_SL_347.raw | Raw | |||
20230614_SL_348.raw | Raw | |||
20230614_SL_349.raw | Raw |
Items per page: 5 1 - 5 of 13 |
Mukhopadhyay Urbi U Levantovsky Sophie S Carusone Teresa Maria TM Gharbi Sarah S Stein Frank F Behrends Christian C Bhogaraju Sagar S
Science advances 20240809 32
Over 600 E3 ligases in humans execute ubiquitination of specific target proteins in a spatiotemporal manner to elicit desired signaling effects. Here, we developed a ubiquitin-specific proximity-based labeling method to selectively biotinylate substrates of a given ubiquitin ligase. By fusing the biotin ligase BirA and an Avi-tag variant to the candidate E3 ligase and ubiquitin, respectively, we were able to specifically enrich bona fide substrates of a ligase using a one-step streptavidin pulld ...[more]