Proteomics

Dataset Information

0

Ubiquitinated proteome of E15.5 murine cortical neurons


ABSTRACT: TRIM9 and TRIM67 are neuronally-enriched E3 ubiquitin ligases essential for neuronal morphogenesis and responses to the axon guidance cue netrin-1. Deletion of either gene in the mouse results in subtle neuroanatomical anomalies yet overt deficits in spatial learning and memory. The identify of few TRIM9 or TRIM67 substrates are known. Here we performed ubiquitin remnant profiling approach (Xu et al., 2010)in cultured cortical neurons from murine wildtype, Trim9-/-, Trim67-/-, and Trim9-/-:Trim67-/- embryos cultured with or without netrin-1 supplementation to attempt to identify proteins with altered ubiquitination. Although batch variability hindered our ability to identify differential protein ubiquitination, the results delineate the neuronal ubiquitionome during neurite outgrowth, axon specification, and axon branching.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Neuron, Primary Cell

SUBMITTER: Stephanie Gupton  

LAB HEAD: Stephanie L. Gupton

PROVIDER: PXD021818 | Pride | 2021-01-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
GU039_WT_digly_Shalini_9ul.raw Raw
GU040_WT_netrin_digly_Shalini_9ul.raw Raw
GU041_TRIM9_digly_Shalini_9ul.raw Raw
GU042_TRIM9_netrin_digly_Shalini_9ul.raw Raw
GU043_TRIM67_digly_Shalini_9ul.raw Raw
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Publications

The ubiquitylome of developing cortical neurons.

Menon Shalini S   Goldfarb Dennis D   Cousins Emily M EM   Major M Ben MB   Gupton Stephanie L SL  

microPublication biology 20201128


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