Dataset Information

Meta-Proteomics profiling of a four species biofilm

ABSTRACT: Microbial biofilms are omnipresent and implicated in a wide spectrum of areas ranging from bioremediation, food production and biomedical applications. To date little is understood about how biofilm communities develop and function on a molecular level, due to the complexity of these biological systems. Here we ap-ply a meta-proteomics approach to investigate the mechanism driving biofilm formation in a microbial model consortium of four bacterial soil isolates of Steno-trophomonas rhizophila, Xanthomonas retroflexus, Microbacterium oxydans and Paeni-bacillus amylolyticus. The protein abundances between community and the single species biofilms were compared to describe how different metabolic pathways were influenced by inter-species interactions. Our results indicate that community development is dependent on interactions between community members facilitat-ing surface attachment and cross-feeding on specific amino acids. Opposite regu-lation patterns of fermentation and nitrogen pathways in Paenibacillus amylolyticus and Xanthomonas retroflexus may, however, also indicate that competition for lim-ited resources affects community development. Overall our results demonstrate the multitude of pathways characterizing biofilm formation in mixed communities. In order to obtain full taxonomic resolution between closely related species and empower correct protein quantification, we developed a novel pipeline for removing peptide sequences shared between community members from the ref-erence proteomes used for spectral database searches. This pipeline can readily be applied to other microbial communities.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Xanthomonas Retroflexus Paenibacillus Amylolyticus Microbacterium Oxydans Stenotrophomonas Rhizophila

SUBMITTER: Jakob Herschend

LAB HEAD: Mette Burmølle

PROVIDER: PXD006281 | Pride | 2017-12-04

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20160412_10418008_ES803_ZD_JHmetaprot_Mix_biorep1_Elution1_01.raw	Raw
	20160412_10418008_ES803_ZD_JHmetaprot_Mix_biorep1_Elution2_01.raw	Raw
	20160412_10418008_ES803_ZD_JHmetaprot_Mix_biorep1_Elution3_01.raw	Raw
	20160412_10418008_ES803_ZD_JHmetaprot_Mix_biorep2_Elution1_01.raw	Raw
	20160412_10418008_ES803_ZD_JHmetaprot_Mix_biorep2_Elution2_01.raw	Raw

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Publications

A meta-proteomics approach to study the interspecies interactions affecting microbial biofilm development in a model community.

Herschend Jakob J Damholt Zacharias B V ZBV Marquard Andrea M AM Svensson Birte B Sørensen Søren J SJ Hägglund Per P Burmølle Mette M

Scientific reports 20171128 1

Microbial biofilms are omnipresent in nature and relevant to a broad spectrum of industries ranging from bioremediation and food production to biomedical applications. To date little is understood about how multi-species biofilm communities develop and function on a molecular level, due to the complexity of these biological systems. Here we apply a meta-proteomics approach to investigate the mechanisms influencing biofilm formation in a model consortium of four bacterial soil isolates; Stenotrop ...[more]

PMID: 29184101

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