Dataset Information

Metabolic profiling of interspecies interactions during sessile bacterial cultivation reveals growth and sporulation induction in Paenibacillus amylolyticus in response to Xanthomonas retroflexus - Samples from Paenibacillus amylolyticus

ABSTRACT: An in-depth characterization of an observed interspecies interaction between two co-isolated bacteria, Xanthomonas retroflexus and Paenibacillus amylolyticus. Using microsensor measurements for mapping the chemical environment, we show how X. retroflexus promoted an alkalization of its local environment through degradation of amino acids and release of ammonia. When the two species were grown in proximity, the modified local environment induced a morphological change and increased growth of P. amylolyticus followed by sporulation. 2D spatial metabolomics enabled visualization and mapping of the degradation of oligopeptide structures by X. retroflexus and morphological changes of P. amylolyticus through e.g. the release of membrane-associated metabolites. Proteome analysis and microscopy were used to validate the shift from vegetative growth towards sporulation. In summary, we demonstrate how environmental profiling by combined application of microsensor, microscopy, metabolomics and proteomics approaches can reveal growth and sporulation promoting effects resulting from interspecies interactions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Paenibacillus Amylolyticus

TISSUE(S): Biofilm

SUBMITTER: Jakob Herschend

LAB HEAD: Mette Burmølle

PROVIDER: PXD030998 | Pride | 2022-05-20

REPOSITORIES: Pride

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			Action	DRS
	20161129_Homepacked20161011_15cm_JakobH_1.raw	Raw
	20161129_Homepacked20161011_15cm_JakobH_10.raw	Raw
	20161129_Homepacked20161011_15cm_JakobH_2.raw	Raw
	20161129_Homepacked20161011_15cm_JakobH_3.raw	Raw
	20161129_Homepacked20161011_15cm_JakobH_4.raw	Raw

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Publications

Metabolic Profiling of Interspecies Interactions During Sessile Bacterial Cultivation Reveals Growth and Sporulation Induction in <i>Paenibacillus amylolyticus</i> in Response to <i>Xanthomonas retroflexus</i>.

Herschend Jakob J Ernst Madeleine M Koren Klaus K Melnik Alexey V AV da Silva Ricardo R RR Røder Henriette L HL Damholt Zacharias B V ZBV Hägglund Per P Svensson Birte B Sørensen Søren J SJ Kühl Michael M Dorrestein Pieter C PC Burmølle Mette M

Frontiers in cellular and infection microbiology 20220329

The toolbox available for microbiologists to study interspecies interactions is rapidly growing, and with continuously more advanced instruments, we are able to expand our knowledge on establishment and function of microbial communities. However, unravelling molecular interspecies interactions in complex biological systems remains a challenge, and interactions are therefore often studied in simplified communities. Here we perform an in-depth characterization of an observed interspecies interacti ...[more]

PMID: 35425721

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