Characterisation of protein ubiquitination using UbiSite technology
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ABSTRACT: We present a novel immune-affinity-based method UbiSite for capture and further identification of ubiquitination sites by MS for any cell line or tissue sample. Implementing the UbiSite methodology for a large-scale experiment we successfully mapped 62000 unique ubiquitination sites on protein substrates for two different human cell lines.
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Hep-2 Cell, Jurkat Cell
SUBMITTER: Iñigo Barrio
LAB HEAD: Blagoy Blagoev
PROVIDER: PXD006299 | Pride | 2018-07-03
REPOSITORIES: Pride
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