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Characterisation of protein ubiquitination using UbiSite technology

ABSTRACT: We present a novel immune-affinity-based method UbiSite for capture and further identification of ubiquitination sites by MS for any cell line or tissue sample. Implementing the UbiSite methodology for a large-scale experiment we successfully mapped 62000 unique ubiquitination sites on protein substrates for two different human cell lines.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hep-2 Cell, Jurkat Cell

SUBMITTER: Iñigo Barrio

LAB HEAD: Blagoy Blagoev

PROVIDER: PXD006299 | Pride | 2018-07-03

REPOSITORIES: Pride

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			Action	DRS
	20160107_QE5_UPLC1_AKP_Hep2_R1_Pro46F_1.raw	Raw
	20160107_QE5_UPLC1_AKP_Hep2_R1_Pro46F_10.raw	Raw
	20160107_QE5_UPLC1_AKP_Hep2_R1_Pro46F_11.raw	Raw
	20160107_QE5_UPLC1_AKP_Hep2_R1_Pro46F_12.raw	Raw
	20160107_QE5_UPLC1_AKP_Hep2_R1_Pro46F_13.raw	Raw

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Characterisation of protein ubiquitination using UbiSite technology

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