Proteomics

Dataset Information

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SUMO targets the APC/C in a phosphorylation-dependent manner to regulate transition from metaphase to anaphase


ABSTRACT: Signal transduction by Small Ubiquitin-like Modifier (SUMO) regulates a myriad of nuclear processes. Here, we report on the role of SUMO in mitosis in human cell lines. Knocking down the SUMO conjugation machinery resulted in a delay in mitosis and defects in mitotic chromosome separation. Searching for relevant SUMOylated proteins in mitosis, we identified the APC/C complex, a master regulator of metaphase to anaphase transition, as a SUMO target. The APC4 subunit is the major SUMO target in the complex, containing SUMO acceptor lysines at positions 772 and 798. SUMOylation increased APC/C ubiquitylation activity towards a subset of its targets, including the newly identified target KIF18B. Intriguingly, SUMOylation of APC/C was regulated by casein kinase driven phosphorylation of serines at positions 777 and 779. Combined, our findings demonstrate the importance of SUMO signal transduction for genome integrity during mitotic progression and reveal a triad of PTMs, cooperating to drive mitosis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sabine Cuijpers  

LAB HEAD: Alfred C.O. Vertegaal

PROVIDER: PXD006335 | Pride | 2018-09-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Filesummary.xlsx Xlsx
peptides-GFP.txt Txt
peptides-endo.txt Txt
peptides-streptactin.txt Txt
proteinGroups-GFP.txt Txt
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Publications

SUMO targets the APC/C to regulate transition from metaphase to anaphase.

Eifler Karolin K   Cuijpers Sabine A G SAG   Willemstein Edwin E   Raaijmakers Jonne A JA   El Atmioui Dris D   Ovaa Huib H   Medema René H RH   Vertegaal Alfred C O ACO  

Nature communications 20180316 1


Signal transduction by small ubiquitin-like modifier (SUMO) regulates a myriad of nuclear processes. Here we report on the role of SUMO in mitosis in human cell lines. Knocking down the SUMO conjugation machinery results in a delay in mitosis and defects in mitotic chromosome separation. Searching for relevant SUMOylated proteins in mitosis, we identify the anaphase-promoting complex/cyclosome (APC/C), a master regulator of metaphase to anaphase transition. The APC4 subunit is the major SUMO tar  ...[more]

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