Metaproteomics of colonic microbiota unveils discrete protein functions among colitic mice and control groups

ABSTRACT: We utilized the well-characterized murine T cell transfer model of colitis to find specific alterations in the intestinal luminal proteome associated with inflammation. Mass spectrometry proteomic analysis of colonic samples permitted the identification of ~10,000-12,000 unique peptides that corresponded to 5610 protein clusters identified across three groups, including the colitic Rag1 -/- T cell recipients, isogenic Rag1 -/- controls, as well as wild-type mice. Bioinformatic analyses on host and microbial proteins found specific proteins and GO term functionalities unique to each group, as well as GO terms shared across the three cohorts. We further demonstrated that the colitic mice exhibited a significant increase in Proteobacteria and Verrucomicrobia that was substantiated with 16S rDNA sequencing.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Human Gut Metagenome

SUBMITTER: Greg Stupp  

LAB HEAD: Dennis Wolan

PROVIDER: PXD006384 | Pride | 2018-07-05

REPOSITORIES: Pride