Proteomics

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“Intelligent versus Blind response”- Unveiling a Hypercellulolytic Fungus Strategy for Efficient Polymeric Carbon Deconstruction via Multiplexed Quantitative Proteomics


ABSTRACT: Fungi are ubiquitous and are often confronted with the need to secure utilisable carbon from their external growth milieu through the use of extracellular proteins to scavenge for carbon from a vast array of complex polymeric carbon sources. This attribute is conserved across evolution in fungi. To understand how filamentous fungi extracellular proteins are modulated in response to the presence polymeric carbons in the environment, we have typed the array of the main extracellular proteins involved and their dynamics using a known hypercellulolytic fungus – Penicillium funiculosum (NCIM 1228), through multiplexed quantitative proteomics

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Talaromyces Funiculosus Ncim1228

SUBMITTER: Funso Ogunmolu  

LAB HEAD: Syed Shams Yazdani

PROVIDER: PXD006445 | Pride | 2018-04-12

REPOSITORIES: Pride

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Publications

Quantitative multiplexed profiling of Penicillium funiculosum secretome grown on polymeric cellulase inducers and glucose.

Ogunmolu Funso Emmanuel FE   Kaur Inderjeet I   Pasari Nandita N   Gupta Mayank M   Yazdani Syed Shams SS  

Journal of proteomics 20180326


Filamentous fungi respond to the need to secure utilisable carbon from their growth milieu by secreting unique extracellular proteins depending upon the types of polymeric substrates. We have here profiled the variations in the secretome pattern of a non-model hypercellulolytic fungus - Penicillium funiculosum, grown in minimal media containing four different polymeric cellulase inducers, i.e., Avicel, wheat bran, ammonium-pretreated wheat straw and Avicel & wheat bran, and glucose over its earl  ...[more]

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