Proteomics

Dataset Information

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Quantitative N-terminome of N-end rule mutant in Arabidopsis

ABSTRACT: According to the /N-end rule pathway, proteins with basic N-termini are targeted for degradation by the Arabidopsis thaliana E3 ligase, PROTEOLYSIS6 (PRT6). Here, we undertook a quantitative proteomics study of N-end rule mutant prt6, to investigate the impact of this pathway on the etiolated seedling. Isolation of N-terminal peptides using terminal amine isotope labelling of samples (TAILS) combined with Tandem mass tag (TMT) identified over 3000 unique N-termini. Trypsin and GluC have advantage of identification of Acetylated or neo-peptides respectively. Seed storage proteins and Cysteine proteins actives are differentially regulated in abundance in the prt6 mutants, which are represent downstream targets of transcription factors known to be N-end rule substrates.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Etiolated Plant Tissue

SUBMITTER: Hongtao Zhang  

LAB HEAD: Hongtao Zhang

PROVIDER: PXD006450 | Pride | 2017-10-27

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
Hongtao-TAILS-TMTsix_combinedallfractionandsinglerun.msf Msf
Pre_T_F_10_04_15.raw Raw
Pre_T_F_10_04_15_fixK.msf Msf
Pre_T_G_10_04_15-02.msf Msf
Pre_T_G_10_04_15.raw Raw
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Publications

N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway.

Zhang Hongtao H   Gannon Lucy L   Hassall Kirsty L KL   Deery Michael J MJ   Gibbs Daniel J DJ   Holdsworth Michael J MJ   van der Hoorn Renier A L RAL   Lilley Kathryn S KS   Theodoulou Frederica L FL  

The New phytologist 20171123 3

The N-end rule pathway of targeted protein degradation is an important regulator of diverse processes in plants but detailed knowledge regarding its influence on the proteome is lacking. To investigate the impact of the Arg/N-end rule pathway on the proteome of etiolated seedlings, we used terminal amine isotopic labelling of substrates with tandem mass tags (TMT-TAILS) for relative quantification of N-terminal peptides in prt6, an Arabidopsis thaliana N-end rule mutant lacking the E3 ligase PRO  ...[more]

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