Dataset Information

Analysis of extracellular matrix proteins from 3D cultures of primary prostate fibroblasts, DU145 cells and mixed cells

ABSTRACT: Composition and organization of extracellular matrix (ECM) in tumor stroma are important regulators of cancer cell behavior. Therefore, ECM produced by cancer associated fibroblasts is often used in in vitro experiments. Here, a proteome wide analysis of ECM proteins in 3D culture is analysed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Prostate Cancer Cell Line, Fibroblast

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Pekka Rappu

LAB HEAD: Jyrki Heino

PROVIDER: PXD006563 | Pride | 2018-03-13

REPOSITORIES: pride

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Publications

The composition of prostate core matrisome in vivo and in vitro unveiled by mass spectrometric analysis.

Ojalill Marjaana M Rappu Pekka P Siljamäki Elina E Taimen Pekka P Boström Peter P Heino Jyrki J

The Prostate 20180309 8

<h4>Background</h4>The composition and organization of extracellular matrix (ECM) are important regulators of cell behavior. In particular in the prostate, this central role of the ECM is further stressed by the fact that several potential markers of prostate stem cells are matrix receptors.<h4>Methods</h4>We established 12 fibroblastic cell lines from cancerous and non-cancerous areas of six prostates and allowed the cells to produce ECM under cell culture conditions. We also performed a proteo ...[more]

PMID: 29520855

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