Dataset Information

Photoperiodic control of the Arabidopsis proteome

ABSTRACT: Plants respond to seasonal cues such as the photoperiod, to adapt to current conditions and to prepare for environmental changes in the season to come. To assess photoperiodic responses at the protein level, we quantified the proteome of the model plant Arabidopsis thaliana by mass spectrometry across four photoperiods. This revealed coordinated changes of abundance in the proteins of photosynthesis, primary metabolism and secondary metabolic processes such as pigment biosynthesis, consistent with higher metabolic activity in long photoperiods. Higher translation rates during the day than during the night likely contribute to these changes, but rhythmic RNA profiles will alter their effects. Photoperiodic control of protein levels might be greatest if high translation rates only coincide with high transcript levels in some photoperiods. We term this mechanism ‘translational coincidence’, mathematically model its components, and demonstrate its effect on the Arabidopsis proteome. Datasets from a green alga and a cyanobacterium suggest that this mechanism is general, contributing to the seasonal control of the proteome in many phototrophic organisms, and favouring RNA rhythms even for stable proteins.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Rosette

SUBMITTER: Alexander Graf

LAB HEAD: Alexander Graf

PROVIDER: PXD006848 | Pride | 2018-02-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20120203_TiMet_Golm_PhotoPer_EON_F1_S31.raw	Raw
	20120203_TiMet_Golm_PhotoPer_EON_F1_S32.raw	Raw
	20120203_TiMet_Golm_PhotoPer_EON_F1_S33.raw	Raw
	20120203_TiMet_Golm_PhotoPer_EON_F1_S34.raw	Raw
	20120203_TiMet_Golm_PhotoPer_EON_F1_S35.raw	Raw

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Photoperiodic control of the <i>Arabidopsis</i> proteome reveals a translational coincidence mechanism.

Seaton Daniel D DD Graf Alexander A Baerenfaller Katja K Stitt Mark M Millar Andrew J AJ Gruissem Wilhelm W

Molecular systems biology 20180301 3

Plants respond to seasonal cues such as the photoperiod, to adapt to current conditions and to prepare for environmental changes in the season to come. To assess photoperiodic responses at the protein level, we quantified the proteome of the model plant <i>Arabidopsis thaliana</i> by mass spectrometry across four photoperiods. This revealed coordinated changes of abundance in proteins of photosynthesis, primary and secondary metabolism, including pigment biosynthesis, consistent with higher meta ...[more]

PMID: 29496885

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