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Proteomics analysis of podocytes with alpha-galactosidase knockdown

ABSTRACT: We subjected human podocytes with and without alpha-Galactosidase knockdown to proteomic analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Markus Rinschen

LAB HEAD: Markus Rinschen

PROVIDER: PXD007080 | Pride | 2018-10-17

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20150430_AWH_FabianBraun_150414_coshRNA-1_01.index	Other
	20150430_AWH_FabianBraun_150414_coshRNA-1_01.raw	Raw
	20150430_AWH_FabianBraun_150414_coshRNA-1_05.index	Other
	20150430_AWH_FabianBraun_150414_coshRNA-1_05.raw	Raw
	20150430_AWH_FabianBraun_150414_coshRNA-1_09.index	Other

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Publications

Urine-derived cells: a promising diagnostic tool in Fabry disease patients.

Slaats Gisela G GG Braun Fabian F Hoehne Martin M Frech Laura E LE Blomberg Linda L Benzing Thomas T Schermer Bernhard B Rinschen Markus M MM Kurschat Christine E CE

Scientific reports 20180723 1

Fabry disease is a lysosomal storage disorder resulting from impaired alpha-galactosidase A (α-Gal A) enzyme activity due to mutations in the GLA gene. Currently, powerful diagnostic tools and in vivo research models to study Fabry disease are missing, which is a major obstacle for further improvements in diagnosis and therapy. Here, we explore the utility of urine-derived primary cells of Fabry disease patients. Viable cells were isolated and cultured from fresh urine void. The obtained cell cu ...[more]

PMID: 30038331

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Deep RNA Sequencing of native mouse podocytes and non-podocytes glomerular cells

Project description:Purpose: Next-generation sequencing (NGS) was used to define the transcriptome of native mouse podocytes and non-podocytes glomerular cells as part of a project aiming to define the molecular fingerprint of mouse podocytes. Method: Glomeruli from 29 Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J x hNPHS2Cre mice at the age of 10 weeks were purified and a single cell solution was prepared to seperate GFP-expressing (podocytes) and GFP-negative (non-podocytes glomerular cells) cells by FACS sorting. RNA was extracted and prepared for further analysis using directional, polyA+ library preparation. An Illumina HiSeq2500 was used for a paired-end sequencing of 100 cycles . Salmon and Sleuth were used for downstream analysis. Results: A total of 100 Million reads each from podocytes and non-podocytes glomerular cells could be used for further analysis.

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Analysis of isotope incorporation in differentiated and undifferentiated podocytes

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Analysis of the cultured podocyte phosphoproteome

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Mechanical stress enhances CD9 expression in cultured podocytes

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Effect of Growth hormone on podocytes

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Genome-wide analysis of WT1-controlled gene expression in podocytes

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