Dataset Information

Co-immumnoprecipitation experiments to identify protein bindining partners of CSDE1

ABSTRACT: While the transcriptional network of human embryonic stem cells (hESCs) has been extensively studied, relatively little is known about how post-transcriptional modulations determine hESC function. RNA-binding proteins play central roles in RNA regulation, including translation and turnover. Here we show that the RNA-binding protein CSDE1 is highly expressed in hESCs to maintain their undifferentiated state and prevent default neural fate. Notably, loss of CSDE1 accelerates neural differentiation and potentiates neurogenesis. Conversely, ectopic expression of CSDE1 impairs neural differentiation. We find that CSDE1 post-transcriptionally modulates core components of multiple regulatory nodes of hESC identity, neuroectoderm commitment and neurogenesis. Among these key pro-neural/neuronal factors, CSDE1 binds fatty acid binding protein 7 (FABP7) and vimentin (VIM) mRNAs as well as transcripts involved in neuron projection development regulating their stability and translation. Thus, our results uncover CSDE1 as a central post-transcriptional regulator of hESC identity and neurogenesis. This proteomics dataset contains the data from co-immunopreciptation experiments using CSDE1 antibody in hESCs

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Embryo, Embryonic Stem Cell

SUBMITTER: David Vilchez

LAB HEAD: David Vilchez

PROVIDER: PXD007271 | Pride | 2018-05-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20150831_UC_Hyun_Vilchez_Control1_CoIP_04.raw	Raw
	20150831_UC_Hyun_Vilchez_Control2_CoIP_05.raw	Raw
	20150831_UC_Hyun_Vilchez_Control3_CoIP_06.raw	Raw
	20150831_UC_Hyun_Vilchez_NT1_CoIP_01.raw	Raw
	20150831_UC_Hyun_Vilchez_NT2_CoIP_02.raw	Raw

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Publications

A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells.

Ju Lee Hyun H Bartsch Deniz D Xiao Cally C Guerrero Santiago S Ahuja Gaurav G Schindler Christina C Moresco James J JJ Yates John R JR Gebauer Fátima F Bazzi Hisham H Dieterich Christoph C Kurian Leo L Vilchez David D

Nature communications 20171113 1

While the transcriptional network of human embryonic stem cells (hESCs) has been extensively studied, relatively little is known about how post-transcriptional modulations determine hESC function. RNA-binding proteins play central roles in RNA regulation, including translation and turnover. Here we show that the RNA-binding protein CSDE1 (cold shock domain containing E1) is highly expressed in hESCs to maintain their undifferentiated state and prevent default neural fate. Notably, loss of CSDE1 ...[more]

PMID: 29129916

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