Proteomics

Dataset Information

0

Substrate identification of ADAMTS16

ABSTRACT: Secreted and cell-surface proteases are major mediators of extracellular matrix remodelling, but their mechanisms and regulatory impact are poorly understood. We developed a mass spectrometry approach using cell free ECM produced by mouse Balb/c 3T3 cells in vitro to identify fibronectin as a novel substrate of the secreted metalloprotease ADAMTS16. ADAMTS16 cleaves fibronectin between its (I)5 and (I)6 modules, releasing the N-terminal 30kDa heparin-binding domain essential for fibronectin assembly.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Permanent Cell Line Cell, Cell Culture, Fibroblast

DISEASE(S): Disease Free

SUBMITTER: Rahel Schnellmann  

LAB HEAD: Suneel S. Apte

PROVIDER: PXD007284 | Pride | 2018-04-23

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
140730mouseMatrix2_ADAMTS16-shsuppl.information_s04.raw Raw
140730mouseMatrix2_AdamTS16-sh-EAsuppl.information_s05.raw Raw
140730mouseMatrix2_vectorsuppl.information_s06.raw Raw
150224_LysC-Tryp_ADAMTS16-sh-EA_s02.raw Raw
150224_LysC-Tryp_ADAMTS16-sh_s01.raw Raw
Items per page:
1 - 5 of 48
altmetric image

Publications

A Selective Extracellular Matrix Proteomics Approach Identifies Fibronectin Proteolysis by A Disintegrin-like and Metalloprotease Domain with Thrombospondin Type 1 Motifs (ADAMTS16) and Its Impact on Spheroid Morphogenesis.

Schnellmann Rahel R   Sack Ragna R   Hess Daniel D   Annis Douglas S DS   Mosher Deane F DF   Apte Suneel S SS   Chiquet-Ehrismann Ruth R  

Molecular & cellular proteomics : MCP 20180418 7

Secreted and cell-surface proteases are major mediators of extracellular matrix (ECM) turnover, but their mechanisms and regulatory impact are poorly understood. We developed a mass spectrometry approach using a cell-free ECM produced <i>in vitro</i> to identify fibronectin (FN) as a novel substrate of the secreted metalloprotease ADAMTS16. ADAMTS16 cleaves FN between its (I)<sub>5</sub> and (I)<sub>6</sub> modules, releasing the N-terminal 30 kDa heparin-binding domain essential for FN self-ass  ...[more]

Similar Datasets

Proteomics Incorporation of chlorophyll a into Rhodobacter sphaeroides
2016-07-12 | PXD002638 | Pride
Proteomics,Multiomics Cell-intrinsic regulation of interneuron migration controls cortical neurogenesis
2018-02-14 | PXD006397 | Pride
Proteomics Caspase-3 cleaves extracellular vesicle proteins during auditory brainstem development
2020-10-16 | PXD021728 | Pride
Proteomics A plasmid goes viral, part 2 - A plasmid from an Antarctic haloarchaeon uses specialized membrane vesicles to disseminate and infect plasmid-free cells
2017-07-14 | PXD006515 | Pride
Proteomics A plasmid goes viral - Analysis of protein compostition of membrane vesicles (MVs), plasmid vesicles (PVs) and membrane fractions of different Halorubrum lacusprofundi strains.
2017-07-11 | PXD005092 | Pride
Proteomics ALOX12-expressing macrophages produce MCTRs to promote local efferocytosis and tissue repair
2023-12-12 | PXD038390 | Pride
Proteomics : Proximity proteomics identifies endothelial cell septins and PAK2 as decisive regulators of actomyosin mediated expulsion of von Willebrand factor.
2023-01-11 | PXD036983 | Pride
Proteomics Spirocyclic dimer (SpiD7) activates unfolded protein response (UPR) to selectively inhibit growth and induce apoptosis of cancer cells
2022-05-20 | PXD029783 | Pride
Proteomics Proteomics exploiting modular baits with different subcellular locations reveal hydrogen peroxide-inducible clone 5 protein (HIC-5) and a glucocorticoid receptor variant as novel testin partners
2018-10-26 | PXD005058 | Pride
Proteomics Ubiquitin mediated intracellular trafficking is required for Jam-C function
2019-11-06 | PXD013003 | Pride