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Characterisation of nuclear and sub-nuclear proteomes of Saccharomyces cerevisiae

ABSTRACT: This study concerns characterisation of the nuclear, chromatin and nuclear non-chromatin proteomes of the yeast Saccharomyces cerevisiae. We performed nuclear and chromatin enrichments from exponentially growing cells using differential centrifugation and analysed the nuclear and chromatin pellets, along with their accompanying supernatants, by LC-MS/MS on two different platforms. We inferred relative quantitation of proteins using emPAI and compared the relative amounts of proteins in each of our samples which enabled us to infer probable residents of the nuclear and chromatin proteomes.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Suspension Culture, Fungal Cell

SUBMITTER: Daniel Nightingale

LAB HEAD: Kathryn Susan Lilley

PROVIDER: PXD007525 | Pride | 2019-11-07

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	ChromatinPellet.dat	Other
	ChromatinPellet.mgf	Mgf
	ChromatinPellet.mzid.gz	Mzid
	ChromatinPellet1.mgf	Mgf
	ChromatinPellet1.raw	Raw

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Publications

Transcriptional regulation of the genes involved in protein metabolism and processing in Saccharomyces cerevisiae.

Dikicioglu Duygu D Nightingale Daniel J H DJH Wood Valerie V Lilley Kathryn S KS Oliver Stephen G SG

FEMS yeast research 20190301 2

Topological analysis of large networks, which focus on a specific biological process or on related biological processes, where functional coherence exists among the interacting members, may provide a wealth of insight into cellular functionality. This work presents an unbiased systems approach to analyze genetic, transcriptional regulatory and physical interaction networks of yeast genes possessing such functional coherence to gain novel biological insight. The present analysis identified only a ...[more]

PMID: 30753445

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