Dataset Information

FANS unfixed: isolation and proteomic analysis of mouse cell type-specific brain nuclei

ABSTRACT: Epigenetic-mediated gene regulation orchestrates brain cell-type gene expression programmes, and epigenetic dysregulation is a major driver of aging and disease-associated changes. Proteins that mediate gene regulation are mostly localised to the nucleus, however, nuclear-localised proteins are often under-represented in gene expression studies and have been understudied in the context of the brain. To address this challenge, we have optimised an approach for nuclei isolation that is compatible with proteomic analysis. This was coupled to a mass spectrometry protocol for detecting proteins in low-concentration samples. We have generated nuclear proteomes for neurons, microglia, and oligodendrocytes from the mouse brain cortex and identified cell-type nuclear proteins associated with chromatin structure and organisation, chromatin modifiers such as transcription factors, and RNA-binding proteins, among others. Our nuclear proteomics platform paves the way for assessing brain cell type changes in the nuclear proteome across health and disease, such as neurodevelopmental, aging, neurodegenerative, and neuroinflammatory conditions.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Harry Whitwell

LAB HEAD: Harry Whitwell

PROVIDER: PXD053515 | Pride | 2024-08-09

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20220520_A_Neurons_Ions.csv	Csv
	20220520_A_Neurons_Peptides.csv	Csv
	20220520_A_Neurons_Proteins.csv	Csv
	20220520_A_Neurons_QC.pdf	Pdf
	20220520_All_Ions.csv	Csv

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Publications

FANS Unfixed: Isolation and Proteomic Analysis of Mouse Cell Type-Specific Brain Nuclei.

Bedwell Lucy L Mavrotas Myrto M Demchenko Nikita N Yaa Reuben M RM Willis Brittannie B Demianova Zuzana Z Syed Nelofer N Whitwell Harry J HJ Nott Alexi A

Journal of proteome research 20240726 9

Epigenetic-mediated gene regulation orchestrates brain cell-type gene expression programs, and epigenetic dysregulation is a major driver of aging and disease-associated changes. Proteins that mediate gene regulation are mostly localized to the nucleus; however, nuclear-localized proteins are often underrepresented in gene expression studies and have been understudied in the context of the brain. To address this challenge, we have optimized an approach for nuclei isolation that is compatible wit ...[more]

PMID: 39056441

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