Proteomics

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Quantitative proteomics and in-cell cross-linking reveal cellular reorganisation during early neuronal differentiation of SH-SY5Y cells


ABSTRACT: The proteomes of undifferentiated and differentiated SH-SY5Y cells are characterised and compared. For this, neuronal differentiation using retinoic acid (RA) or a combination of RA and phorbol-12-myristat-13-acetate (RA/PMA) was explored. An MS-based label-free quantification approach is applied to identify changes in the protein expression, the as proteins’ subcellular localisation abundance as well as their association with enriched KEGG pathways. By employing formaldehyde cross-linking insights into protein interaction networks of undifferentiated as well as RA- and RA/PMA-differentiated neurons are obtained. The analyses provided insights into the proteomes of undifferentiated and differentiated SH-SY5Y cells and suggest structural rearrangements, for instance, of the actin network during neuronal differentiation.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Neuroblastoma

SUBMITTER: Marie Alfes  

LAB HEAD: Carla Schmidt

PROVIDER: PXD031054 | Pride | 2022-04-08

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20201027_LP_MB_SHSY5Y_PMA_R1.raw Raw
20201027_LP_MB_SHSY5Y_PMA_R2.raw Raw
20201027_LP_MB_SHSY5Y_PMA_R3.raw Raw
20201027_LP_MB_SHSY5Y_RA_R1.raw Raw
20201027_LP_MB_SHSY5Y_RA_R2.raw Raw
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