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Hypoxia induces Foxn1 and Mmp-9 expression in keratinocytes: proteomics analysis of mouse keratinocytes transiently activated by Foxn1

ABSTRACT: Recent studies have shown that transcription factor Foxn1 expressed in keratinocytes is involved in skin wound healing process, yet how Foxn1 functions remains largely unknown. Proteomic analysis using a two-dimensional differential gel electrophoresis (2D-DIGE) technique coupled with MALDI-TOF/TOF was used to analyze in vitro cultured keratinocytes transfected with adenoviral vector carrying Foxn1-GFP or GFP-alone (control). Sixty-six of sixty-nine protein spots differed in abundance between compared groups were identified.

INSTRUMENT(S): autoflex

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Keratinocyte

SUBMITTER: Mariola Dietrich

LAB HEAD: Mariola Dietrich

PROVIDER: PXD007843 | Pride | 2018-04-09

REPOSITORIES: Pride

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			Action	DRS
	spot_01.dat	Other
	spot_01.mgf	Mgf
	spot_01.zip	Other
	spot_02.dat	Other
	spot_02.mgf	Mgf

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Publications

Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing.

Kur-Piotrowska Anna A Bukowska Joanna J Kopcewicz Marta M MM Dietrich Mariola M Nynca Joanna J Slowinska Mariola M Gawronska-Kozak Barbara B

Scientific reports 20180403 1

Recent studies have shown that the transcription factor Foxn1, which is expressed in keratinocytes, is involved in the skin wound healing process, yet how Foxn1 functions remains largely unknown. Our latest data indicate that Foxn1 drives skin healing via engagement in re-epithelization and the epithelial-mesenchymal transition (EMT) process. In the present study, 2D-DIGE proteomic profiling analysis of in vitro cultured keratinocytes transfected with adenoviral vector carrying Foxn1-GFP or GFP ...[more]

PMID: 29615703

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