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Proteomic studies on the swimbladder of the European eel (Anguilla anguilla)

ABSTRACT: The swimbladder of fish is a vital organ that along with gas glands in the swimbladder wall enables key physiological functions including buoyance, diving and coping with different hydrostatic pressures. Specific intramural glands provide gas for regulating gas pressure in the swimming bladder. Transcriptomic analysis have revealed evidence at the RNA level but no specific studies at the protein level have been carried out so far. Herein, it was the aim of the study to show swimbladder proteins of the European eel by label-free LC/MS (Q-Exactive Plus) that resulted in the identification of 6,223 protein groups. Neurotransmitter receptors and -transporters were enriched in the membrane fraction and enzymes for gas production were observed. The list of identified proteins may represent a useful tool for further proteomics experiments on this organ.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Anguilla Anguilla

TISSUE(S): Swim Bladder

SUBMITTER: Fernando J. Sialana

LAB HEAD: Gert Lubec

PROVIDER: PXD007850 | Pride | 2018-02-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Eel-swimbladder-biochemicalfractionation.msf	Msf
	Eel-swimbladder-proteome.msf	Msf
	Eel_cDNA_20170720.fasta	Fasta
	NM04-TSBcyt-BA4-P1360-1.raw	Raw
	NM04-TSBcyt-BA5-P1361-1.raw	Raw

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Project description:Treatment of hematological malignancies by adoptive transfer of activated natural killer (NK) cells is limited by poor post-infusion persistence. We compared the ability of interleukin-2 (IL-2) and IL-15 to sustain human NK cell functions following cytokine withdrawal to model post-infusion performance. In contrasts to IL-2, IL-15 mediated stronger signaling through the IL-2/15 receptor complex and provided functional advantages. Genome-wide analysis of cytosolic and polysome-associated mRNA revealed cytokine dependent differential mRNA levels and translation during cytokine activation but also that most gene expression differences were primed by IL-15 and only manifested after cytokine withdrawal. IL-15 augmented mTOR signaling, which correlated with increased expression of genes related to cell metabolism and respiration. Consistently, mTOR inhibition abrogated IL-15-induced functional advantages. Moreover, mTOR-independent STAT-5 signaling contributed to improved NK cell function during cytokine activation but not following cytokine withdrawal. The superior performance of IL-15 stimulated NK cells was also observed using a clinically applicable protocol for NK cell expansion. Finally, expression of IL-15 correlated with cytolytic immune functions in patients with B cell lymphoma and favorable clinical outcome. These findings highlight the importance of mTOR regulated metabolic processes for immune cell functions and argue for implementation of IL-15 in adoptive NK cell cancer therapy. Freshly isolated NK cells from 6 donors were activated with IL-2 or IL-15 for 48 hours, followed by cytokine withdrawal for 24 hours, resulting in four RNA samples per donor. From each sample, both the cytosolic as well as the polysomal fraction were collected. Donor 3 contains activation and post withdrawal data from two different donors due to poor RNA-quality obtained for some samples which did not allow for processing of the complete set of 6 donors (resulting in a total of 40 samples).

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Proteomic studies on the swimbladder of the European eel (Anguilla anguilla)

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