Dataset Information

A secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cell line

ABSTRACT: Increased fatty acid (FA) is often observed in highly proliferative tumors, contributing to promoting proliferation of tumor cells. FA affects secreted factors from tumor cells, which can modulate tumor microenvironment in favor of tumor survival. However, the secreted factors affected by the increased FA have not been systematically explored. Here, we performed comprehensive secretome profiling of oleate-treated and untreated HepG2 cells. Comparison of the secretomes identified 349 differentially secreted proteins (DSPs; 145 up-regulated and 192 down-regulated) in oleate-treated samples, compared to untreated samples. Functional enrichment and network analyses of the DSPs revealed that the 145 up-regulated secreted proteins by oleate treatment were mainly associated with cell proliferation-related processes, such as lipid metabolism, inflammatory response, and ER stress. Based on the network models of the DSPs, we selected four up-regulated secreted proteins (MIF, THBS1, PDIA3, and APOA1) that can represent such processes related to cell proliferation. Thus, our results provided a secretome profile indicative of oleate-induced proliferation of HepG2 cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Ji-Hwan Park

LAB HEAD: Daehee Hwang

PROVIDER: PXD007906 | Pride | 2018-08-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PSY_NT_CM1_20131113_1_PEMMR.mgf	Mgf
	PSY_NT_CM1_20131113_2_PEMMR.mgf	Mgf
	PSY_NT_CM1_20131113_3_PEMMR.mgf	Mgf
	PSY_NT_CM1_A.raw	Raw
	PSY_NT_CM1_A_PEMMR.mzid.gz	Mzid

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A secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cells.

Park Soyeon S Park Ji-Hwan JH Jung Hee-Jung HJ Jang Jin-Hyeok JH Ahn Sanghyun S Kim Younah Y Suh Pann-Ghill PG Chae Sehyun S Yoon Jong Hyuk JH Ryu Sung Ho SH Hwang Daehee D

Experimental & molecular medicine 20180803 8

Increased fatty acid (FA) is often observed in highly proliferative tumors. FAs have been shown to modulate the secretion of proteins from tumor cells, contributing to tumor survival. However, the secreted factors affected by FA have not been systematically explored. Here, we found that treatment of oleate, a monounsaturated omega-9 FA, promoted the proliferation of HepG2 cells. To examine the secreted factors associated with oleate-induced cell proliferation, we performed a comprehensive secret ...[more]

PMID: 30076294

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