Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Permanent Cell Line Cell
SUBMITTER: Tetsuya Hirata
LAB HEAD: Taroh Kinoshita
PROVIDER: PXD008137 | Pride | 2018-01-30
REPOSITORIES: Pride
Items per page: 5 1 - 5 of 14 |
Hirata Tetsuya T Mishra Sushil K SK Nakamura Shota S Saito Kazunobu K Motooka Daisuke D Takada Yoko Y Kanzawa Noriyuki N Murakami Yoshiko Y Maeda Yusuke Y Fujita Morihisa M Yamaguchi Yoshiki Y Kinoshita Taroh T
Nature communications 20180126 1
Many eukaryotic proteins are anchored to the cell surface via the glycolipid glycosylphosphatidylinositol (GPI). Mammalian GPIs have a conserved core but exhibit diverse N-acetylgalactosamine (GalNAc) modifications, which are added via a yet unresolved process. Here we identify the Golgi-resident GPI-GalNAc transferase PGAP4 and show by mass spectrometry that PGAP4 knockout cells lose GPI-GalNAc structures. Furthermore, we demonstrate that PGAP4, in contrast to known Golgi glycosyltransferases, ...[more]