Proteomics

Dataset Information

0

Identification of interaction partners of the Toxoplasma gondii Cysteine Repeat Modular Proteins TGGT1_292020 and TGGT1_261080


ABSTRACT: Apicomplexa are obligate intracellular parasites. While most species are restricted to specific hosts and cell types, Toxoplasma gondii can invade every nucleated cell derived from warm-blooded animals. This broad host range suggests that this parasite can recognize multiple host cell ligands or structures, leading to the activation of a central protein complex, which should be conserved in all apicomplexans. Cysteine Repeat Modular Proteins (CRMPs) are a family of apicomplexan specific proteins. In Toxoplasma gondii, two CRMPs are present in the genome. We performed pulldown of 3xHA tagged CRMPA and CRMPB. In a second dataset we performed biotinylation of TurboID tagged CRMPB on extracellular parasites to identify transient interaction partners. In a third dataset, we performed biotinylation of TurboID tagged CRMPA or B during invasion or in an invasion blocking buffer.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Toxoplasma Gondii Rh

DISEASE(S): Toxoplasmosis

SUBMITTER: Ignasi Forne  

LAB HEAD: Prof. Dr. rer. nat. Markus Meissner

PROVIDER: PXD031649 | Pride | 2022-12-13

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Groups_Ref3590_S01-S05.txt Txt
Groups_Ref3592_S01-S02_S04-S07.txt Txt
Groups_Ref6891_S03-S14.txt Txt
Ref3590_MS_01_20201119.raw Raw
Ref3590_MS_02_20201119.raw Raw
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