Multi-laboratory analysis of sample stability for proteomics following non-cooled international transport

ABSTRACT: As international collaborative research grows there is increasing desire to transport specimens around the globe. Transporting biological samples such as cells or tissues is complicated by the need to maintain integrity and minimise modification and degradation, but this is economically costly as the samples must be shipped in a frozen state. This multi-laboratory study investigated whether non-cooled transport of dried peptide samples maintained stability for proteomic analysis using mass spectrometry. Human cancer cell lysates were proteolysed and dried in Australia and shipped by air to Europe and China for label free mass spectrometry using similar LC-MS systems. Preparation and analysis of the specimens in this manner resulted in only minor differences in protein identification and high quantitative reproducibility amongst the participating laboratories. We examined any impact on peptide chemical modification and report no discrepancies compared to the starting, non-shipped sample. We conclude that the transport of non-cooled, dried peptides has little to no effect on sample integrity for downstream LC-MS analysis and therefore represents a cost-effective option to facilitate international proteomic collaborations.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Colon Epithelial Cell

DISEASE(S): Colon Cancer

SUBMITTER: Pascal Steffen  

LAB HEAD: Hartmut Schlueter

PROVIDER: PXD008160 | Pride | 2018-03-02

REPOSITORIES: Pride