Dataset Information

Proteomic approach for the analysis response of macrophages to Borrelia burgdorferi

ABSTRACT: Macrophages are cells of the innate immune system with the ability to phagocytose and induce a global pattern of responses that depend on several signalling pathways. In this work we tried to uncover the biosignature of murine bone marrow-derived macrophages and human blood monocytes upon Borrelia infection using transcriptomics and proteomics approaches.

OTHER RELATED OMICS DATASETS IN: GSE103483PRJNA401784PRJNA401425PRJNA401732

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Borrelia Sp. Bc7 Mus Musculus (mouse)

TISSUE(S): Macrophage

DISEASE(S): Lyme Disease

SUBMITTER: Mikel Azkargorta

LAB HEAD: Felix Elortza

PROVIDER: PXD008228 | Pride | 2018-10-22

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 18

Publications

A multi-omic analysis reveals the regulatory role of CD180 during the response of macrophages to Borrelia burgdorferi.

Carreras-González Ana A Navasa Nicolás N Martín-Ruiz Itziar I Lavín José Luis JL Azkargorta Mikel M Atondo Estíbaliz E Barriales Diego D Macías-Cámara Nuria N Pascual-Itoiz Miguel Angel MA Sampedro Leticia L Tomás-Cortázar Julen J Peña-Cearra Ainize A Pellón Aize A Prados-Rosales Rafael R Abecia Leticia L Elortza Félix F Aransay Ana M AM Rodríguez Héctor H Anguita Juan J

Emerging microbes & infections 20180307 1

Macrophages are cells of the innate immune system with the ability to phagocytose and induce a global pattern of responses that depend on several signaling pathways. We have determined the biosignature of murine bone marrow-derived macrophages and human blood monocytes using transcriptomic and proteomic approaches. We identified a common pattern of genes that are transcriptionally regulated and overall indicate that the response to B. burgdorferi involves the interaction of spirochetal antigens ...[more]

PMID: 29511161

Similar Datasets

Project description:Borrelia burgdorferi and Borrelia miyamotoi are tick-vectored zoonotic pathogens maintained in wildlife species. Tick populations are establishing in new areas globally in response to climate change and other factors. New Brunswick is a Canadian maritime province at the advancing front of tick population establishment and has seen increasing numbers of ticks carrying B. burgdorferi, and more recently B. miyamotoi. Further, it is part of a region of Atlantic Canada with wildlife species composition differing from much of continental North America and little information exists as to the presence and frequency of infection of Borrelia spp. in wildlife in this region. We used a citizen science approach to collect a wide range of animals including migratory birds, medium-sized mammals, and small mammals. In total we tested 339 animals representing 20 species for the presence of B. burgdorferi and B. miyamotoi. We have developed new nested PCR primers and a protocol with excellent specificity for detecting both of these Borrelia species, both single and double infections, in tissues and organs of various wildlife species. The positive animals were primarily small non-migratory mammals, approximately twice as many were infected with B. burgdorferi than B. miyamotoi and one animal was found infected with both. In addition to established reservoir species, the jumping mouse (Napaeozapus insignis) was found frequently infected; this species had the highest infection prevalence for both B. burgdorferi and B. miyamotoi and has not previously been identified as an important carrier for either Borrelia species. Comprehensive testing of tissues found that all instances of B. burgdorferi infection were limited to one tissue within the host, whereas two of the five B. miyamotoi infections were diffuse and found in multiple systems. In the one coinfected specimen, two fetuses were also recovered and found infected with B. miyamotoi. This presumptive transplacental transmission suggests that vertical transmission in mammals is possible. This finding implies that B. miyamotoi could rapidly spread into wildlife populations, as well as having potential human health implications.

			Action	DRS
	10_NICO_WT_Bb1-01.msf	Msf
	10_NICO_WT_Bb1.msf	Msf
	10_NICO_WT_Bb1.raw	Raw
	11_NICO_WT_bb2-01.msf	Msf
	11_NICO_WT_bb2.msf	Msf