Proteomics

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Histone-like family of nucleoid structuring protein of A. actinomycetemcomitans promotes biofilm formation and alters protein dynamics of other species within multi-species biofilm


ABSTRACT: Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. An important virulence property of A. actinomycetemcomitans is its ability to form tenacious biofilms that can attach to abiotic as well as biotic surfaces. The histone-like (H-NS) family of nucleoid structuring proteins act as transcriptional silencers in many Gram-negative bacteria. To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of the serotype a strain D7S were generated. Characteristics of the hns mutant phenotype included shorter and fewer pili, which were accompanied by substantially lower biofilm formation relative to the wild type. In accordance with this observation, the D7S hns mutant exhibited significantly reduced growth within a seven-species biofilm model. However, no apparent difference was observed regarding the numbers and proportions of the remaining six species in the biofilm regardless co-cultivated with D7S hns or its parental strain. Proteomics analysis of the strains grown in monocultures confirmed the role of H-NS as a repressor of gene expression in A. actinomycetemcomitans. Interestingly, proteomics analysis of the multispecies biofilms indicated that the A. actinomycetemcomitans wildtype and hns mutant imposed different regulatory effects on the pattern of protein expression in the other species, i.e. mainly Streptococcus spp., Fusobacterium nucleatum, and Veillonella dispar. Gene ontology analysis revealed that a large portion of the differentially regulated proteins was related to translational activity. Taken together, our data suggest that, apart from being a negative regulator of gene expression in A. actinomycetemcomitans, H-NS promotes biofilm formation and may be an important factor for survival of this species within a multispecies biofilm.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Aggregatibacter Aphrophilus Streptococcus Mutans Streptococcus Oralis Fusobacterium Nucleatum Veillonella Dispar Candida Albicans (yeast) Actinomyces Oris Aggregatibacter Actinomycetemcomitans (actinobacillus Actinomycetemcomitans) (haemophilus Actinomycetemcomitans)

TISSUE(S): Biofilm

SUBMITTER: Kai Bao  

LAB HEAD: Nagihan Bostanci

PROVIDER: PXD008444 | Pride | 2018-06-05

REPOSITORIES: pride

Dataset's files

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Action DRS
20170111_02_227_3_10.raw Raw
20170111_04_227_3_19.raw Raw
20170111_06_227_2_34.raw Raw
20170111_07_227_3_18.raw Raw
20170111_11_227_3_9.raw Raw
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<i>Aggregatibacter actinomycetemcomitans</i> H-NS promotes biofilm formation and alters protein dynamics of other species within a polymicrobial oral biofilm.

Bao Kai K   Bostanci Nagihan N   Thurnheer Thomas T   Grossmann Jonas J   Wolski Witold E WE   Thay Bernard B   Belibasakis Georgios N GN   Oscarsson Jan J  

NPJ biofilms and microbiomes 20180522


<i>Aggregatibacter actinomycetemcomitans</i> is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. An important virulence property of <i>A. actinomycetemcomitans</i> is its ability to form tenacious biofilms that can attach to abiotic as well as biotic surfaces. The histone-like (H-NS) family of nucleoid-structuring proteins act as transcriptional silencers in many Gram-negative bacteria. To evaluate the role of H-NS in <i>A. actinomycetemcomitans</i>, <i>hns</  ...[more]

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