Dataset Information

MacAB-TolC, a determinant of solid-liquid biofilms in Acinetobacter baumannii

ABSTRACT: Acinetobacter baumannii has emerged as one of the most problematic opportunist bacterial pathogen responsible for hospital-acquired and community infections worldwide. Besides its high capacities to acquire antibiotic resistance mechanisms, it also presents high adhesion abilities on inert and living surfaces leading to biofilm development, a lifestyle conferring an additional protection against various treatments, and allowing it to persist for long periods in various hospital niches. Due to their increasing resilience to antimicrobial treatments, A. baumannii biofilms are difficult to control and ultimately eradicate. Overcoming the significant challenges pose by A. baumannii require fundamental insights into mechanisms involved in the development of mature biofilm and thus, may help to develop novel strategies for biofilm prevention and control. To unravel critical determinants of this sessile lifestyle, we compared the proteome profiles of bacteria grown in planktonic stationary phase with those of two A. baumannii strains (ATCC 17978 and SDF), harboring specific features regarding biofilm formation, grown in mature solid-liquid (S-L) biofilm using a proteomic quantitative study. Of interest, among the 69 common proteins determinants accumulated in the two strains at the S-L interface, we sorted out the MacAB-TolC system. This tripartite efflux pump appears to play a role in A. baumannii biofilm formation as demonstrated by using ΔmacAB-tolC deletion mutant. Complementary approaches allowed us to get an overview of the impact of macAB-tolC deletion in A. baumannii physiology. Indeed, this efflux pump appeared to be involved in the envelope stress response occurring in mature biofilm and contributes to maintain WT membrane rigidity as well as tolerance to high osmolarity conditions. In addition, this system is probably involved in the maintenance of iron and sulfur homeostasis. MacAB-TolC might help this pathogen facing and adapting to biofilm architecture which is heterogeneous in space and time, especially in mature biofilm. Increasing our knowledge of A. baumannii biofilm formation will undoubtedly help us develop new therapeutic strategies to tackle this emerging threat to human health.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Acinetobacter Baumannii

SUBMITTER: Julie Hardouin

LAB HEAD: Emmanuelle Dé

PROVIDER: PXD028619 | Pride | 2022-02-16

REPOSITORIES: Pride

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			Action	DRS
	ATCC_BioF_24H_Memb_2prime_01.raw	Raw
	ATCC_BioF_24H_Memb_3_01.raw	Raw
	ATCC_BioF_24H_Memb_3prime_01.raw	Raw
	ATCC_BioF_24H_Memb_4_01.raw	Raw
	ATCC_BioF_24H_Memb_4prime_01.raw	Raw

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Publications

MacAB-TolC Contributes to the Development of <i>Acinetobacter baumannii</i> Biofilm at the Solid-Liquid Interface.

Robin Brandon B Nicol Marion M Le Hung H Tahrioui Ali A Schaumann Annick A Vuillemenot Jean-Baptiste JB Vergoz Delphine D Lesouhaitier Olivier O Jouenne Thierry T Hardouin Julie J Potron Anaïs A Perrot Valérie V Dé Emmanuelle E

Frontiers in microbiology 20220113

<i>Acinetobacter baumannii</i> has emerged as one of the most problematic bacterial pathogens responsible for hospital-acquired and community infections worldwide. Besides its high capacity to acquire antibiotic resistance mechanisms, it also presents high adhesion abilities on inert and living surfaces leading to biofilm development. This lifestyle confers additional protection against various treatments and allows it to persist for long periods in various hospital niches. Due to their remarkab ...[more]

PMID: 35095797

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