Proteomics

Dataset Information

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Microbial Interactions Underpinning Persistence Across Hydraulically Fractured Shales

ABSTRACT: Investigating microbial community interactions across hydraulically fractured shales

INSTRUMENT(S): Q Exactive

ORGANISM(S): Unclassified Bacteria

SUBMITTER: Matthew Monroe  

LAB HEAD: Kelly Wrighton

PROVIDER: PXD008490 | Pride | 2018-10-22

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
OSU_A2_TF_GB_12_run1_22Jul16_Samwise_16-05-02.mgf Mgf
OSU_A2_TF_GB_12_run1_22Jul16_Samwise_16-05-02.pride.mgf.gz Mgf
OSU_A2_TF_GB_12_run1_22Jul16_Samwise_16-05-02.raw Raw
OSU_A2_TF_GB_12_run1_22Jul16_Samwise_16-05-02_msgfplus.mzid.gz Mzid
OSU_A2_TF_GB_12_run1_22Jul16_Samwise_16-05-02_msgfplus.pride.mztab.gz Mztab
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Publications

Coupled laboratory and field investigations resolve microbial interactions that underpin persistence in hydraulically fractured shales.

Borton Mikayla A MA   Hoyt David W DW   Roux Simon S   Daly Rebecca A RA   Welch Susan A SA   Nicora Carrie D CD   Purvine Samuel S   Eder Elizabeth K EK   Hanson Andrea J AJ   Sheets Julie M JM   Morgan David M DM   Wolfe Richard A RA   Sharma Shikha S   Carr Timothy R TR   Cole David R DR   Mouser Paula J PJ   Lipton Mary S MS   Wilkins Michael J MJ   Wrighton Kelly C KC  

Proceedings of the National Academy of Sciences of the United States of America 20180625 28

Hydraulic fracturing is one of the industrial processes behind the surging natural gas output in the United States. This technology inadvertently creates an engineered microbial ecosystem thousands of meters below Earth's surface. Here, we used laboratory reactors to perform manipulations of persisting shale microbial communities that are currently not feasible in field scenarios. Metaproteomic and metabolite findings from the laboratory were then corroborated using regression-based modeling per  ...[more]

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