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Human primary brown and white fat cell secretome

ABSTRACT: Secreted proteins from adipose tissue play a role in metabolic cross-talk and homeostasis. We performed high sensitivity mass spectrometry-based proteomics on the cell media of human adipocytes derived from the supraclavicular brown adipose and from the subcutaneous white adipose depots of adult humans. We identified 471 potentially secreted proteins covering interesting protein categories such as hormones, growth factors, extracellular matrix proteins and proteins of the complement system, which were differentially regulated in brown and white adipocytes. A total of 101 proteins were exclusively quantified in brown adipocytes and among these were ependymin-related protein 1 (EPDR1). Functional studies suggested a role for EPDR1 in thermogenic adipogenesis. In conclusion, we report substantial differences between the secretomes of brown and white human adipocytes and identify novel candidate batokines that can be important regulators of metabolism.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): White Fat Cell, Brown Adipose Tissue, White Adipose Tissue, Brown Fat Cell

SUBMITTER: atul shahaji deshmukh

LAB HEAD: Matthias Mann

PROVIDER: PXD008541 | Pride | 2019-10-03

REPOSITORIES: Pride

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			Action	DRS
	20140404_EXQ8_AtDe_SA_WAT_Secr_MC17_wo_NE4.raw	Raw
	20140404_EXQ8_AtDe_SA_WAT_Secr_MC18_NE4.raw	Raw
	20140404_EXQ8_AtDe_SA_WAT_Secr_MC1_wo_NE3.raw	Raw
	20140404_EXQ8_AtDe_SA_WAT_Secr_MC29_wo_NE5.raw	Raw
	20140404_EXQ8_AtDe_SA_WAT_Secr_MC2_NE3.raw	Raw

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