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Dissection of affinity captured LINE-1 macromolecular complexes

ABSTRACT: Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we have characterized the proteins and nucleic acids associated with distinctive, enzymatically active L1 macromolecular complexes. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNA-containing RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in target-primed reverse transcription.

OTHER RELATED OMICS DATASETS IN: PRJNA423015

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ilya Altukhov

LAB HEAD: John Paul LaCava

PROVIDER: PXD008542 | Pride | 2018-01-17

REPOSITORIES: Pride

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			Action	DRS
	Human_041316_UP000005640_9606.fasta	Fasta
	KM010813_SILAC_F.RAW	Raw
	KM010913_SILAC_MW.RAW	Raw
	KM012714_RNAseSILAC_3.raw	Raw
	KM013114_SILACRNAse_4.raw	Raw

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