Dataset Information

Proteomics of human cord blood reticulocyte-derived exosomes

ABSTRACT: Human reticulocytes were enriched from human umbilical blood, and cultured in vitro for the production of reticulocyte-derived exosomes (HuRex)to unveil their proteomic composition. As research on the molecular-cargo of exosomes is largely confounded by the lack of a “gold-standard” methodology, we applied several approaches for obtaining HuRex prior to MS. HuRex preparations were obtained from cultures in absence of serum (AS) from three donors and isolated by means of size-exclusion chromatography (SEC) (n=3) and ultracentrifugation (UC) (n=3). HuRex preparations in the presence of serum were obtained from three other donors and purified by SEC (n=3) and UC (n=1).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hernando A del Portillo

LAB HEAD: Hernando A. del Portillo

PROVIDER: PXD008545 | Pride | 2018-09-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	170607_S_JSHP_01_01_1ug.msf	Msf
	170607_S_JSHP_01_01_1ug.raw	Raw
	170607_S_JSHP_02_01_1ug.msf	Msf
	170607_S_JSHP_02_01_1ug.raw	Raw
	170607_S_JSHP_03_01_1ug.msf	Msf

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Publications

Proteomics study of human cord blood reticulocyte-derived exosomes.

Díaz-Varela Míriam M de Menezes-Neto Armando A Perez-Zsolt Daniel D Gámez-Valero Ana A Seguí-Barber Joan J Izquierdo-Useros Nuria N Martinez-Picado Javier J Fernández-Becerra Carmen C Del Portillo Hernando A HA

Scientific reports 20180919 1

Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex (HuRex). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, ...[more]

PMID: 30232403

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