Dataset Information

ITRAQ-based quantitative phosphoproteomic analysis of a prp4ka mutant in Arabidopsis thaliana

ABSTRACT: We identified PRP4 kinase-A (PRP4ka) in a forward genetic screen based on an alternatively-spliced GFP reporter gene in Arabidopsis thaliana (Arabidopsis). Prp4 kinase, which was the first spliceosome-associated kinase shown to regulate splicing in fungi and mammals, has not yet been studied in plants. Analysis of RNA-seq data from the prp4ka mutant revealed widespread perturbations in alternative splicing. A quantitative iTRAQ-based phosphoproteomics investigation of the mutant identified phosphorylation changes in several serine/arginine-rich proteins, which regulate constitutive and alternative splicing, as well as other splicing-related factors. The results demonstrate the importance of PRP4ka in alternative splicing and suggest that PRP4ka may influence alternative splicing patterns by phosphorylating a subset of splicing regulators.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Seedling

SUBMITTER: Tuan-Nan Wen

LAB HEAD: Antonius Matzke

PROVIDER: PXD008580 | Pride | 2018-10-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	R327_prp4ka-4_AtRTD2_iTRAQ2plex_Phospho_Bio1.rar	Other
	R327_prp4ka-4_AtRTD2_iTRAQ2plex_Phospho_Bio1_20180301.mzML.gz	Mzml
	R327_prp4ka-4_AtRTD2_iTRAQ2plex_Phospho_Bio1_20180522.msf.gz	Msf
	R327_prp4ka-4_AtRTD2_iTRAQ2plex_Phospho_Bio2.rar	Other
	R327_prp4ka-4_AtRTD2_iTRAQ2plex_Phospho_Bio2_20180304.mzML.gz	Mzml

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Publications

PRP4KA, a Putative Spliceosomal Protein Kinase, Is Important for Alternative Splicing and Development in <i>Arabidopsis thaliana</i>.

Kanno Tatsuo T Venhuizen Peter P Wen Tuan-Nan TN Lin Wen-Dar WD Chiou Phebe P Kalyna Maria M Matzke Antonius J M AJM Matzke Marjori M

Genetics 20181008 4

Splicing of precursor messenger RNAs (pre-mRNAs) is an essential step in the expression of most eukaryotic genes. Both constitutive splicing and alternative splicing, which produces multiple messenger RNA (mRNA) isoforms from a single primary transcript, are modulated by reversible protein phosphorylation. Although the plant splicing machinery is known to be a target for phosphorylation, the protein kinases involved remain to be fully defined. We report here the identification of pre-mRNA proces ...[more]

PMID: 30297453

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