Dataset Information

Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis

ABSTRACT: Aggregation is a sequence-specific process, nucleated by short aggregation prone regions (APR) that can be exploited to induce aggregation of proteins containing the same APR. Here we find that most APRs are unique within a proteome, but that a small minority of APRs occur in many proteins. When aggregation is nucleated in bacteria by such frequently occurring APRs, it leads to massive and lethal inclusion body formation containing a large number of proteins. Build-up of bacterial resistance against these peptides is slow. In addition, the approach is effective against drug-resistant clinical isolates of E. coli and A. baumannii, reducing bacterial load in a murine bladder infection model. Our results indicate that redundant APRs are weak points of bacterial protein homeostasis and that targeting these may be an attractive antibacterial strategy.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli

SUBMITTER: Ladan Khodaparast

LAB HEAD: Joost Schymkowitz

PROVIDER: PXD008685 | Pride | 2018-03-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20160121_laleh_ladan_sample_1.raw (F020863).mzid_20160121_laleh_ladan_sample_1.raw_(F020863).pride.mgf.gz	Mzid
	20160121_laleh_ladan_sample_1.raw (F020863).pride.mztab.gz	Mztab
	20160121_laleh_ladan_sample_1.rawF020863.mzid.gz	Mzid
	20160121_laleh_ladan_sample_1.rawF020863.mzid_20160121_laleh_ladan_sample_1.raw_F020863.MGF	Mzid
	20171003_laleh_ladan_sample_003 (F025043).mzid_20171003_laleh_ladan_sample_003_(F025043).pride.mgf.gz	Mzid

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Publications

Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis.

Khodaparast Ladan L Khodaparast Laleh L Gallardo Rodrigo R Louros Nikolaos N NN Michiels Emiel E Ramakrishnan Reshmi R Ramakers Meine M Claes Filip F Young Lydia L Shahrooei Mohammad M Wilkinson Hannah H Desager Matyas M Mengistu Tadesse Wubishet W Nilsson K Peter R KPR Hammarström Per P Aertsen Abram A Carpentier Sebastien S Van Eldere Johan J Rousseau Frederic F Schymkowitz Joost J

Nature communications 20180228 1

Aggregation is a sequence-specific process, nucleated by short aggregation-prone regions (APRs) that can be exploited to induce aggregation of proteins containing the same APR. Here, we find that most APRs are unique within a proteome, but that a small minority of APRs occur in many proteins. When aggregation is nucleated in bacteria by such frequently occurring APRs, it leads to massive and lethal inclusion body formation containing a large number of proteins. Buildup of bacterial resistance ag ...[more]

PMID: 29491361

Dataset Information

Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis

Dataset's files

Publications

Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis.

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