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Membrane protein interactome - A proximity-tagging system to identify membrane protein-protein interactions

ABSTRACT: we have developed a new catalytic tagging system and have used it on the identification of interacting partners of membrane proteins

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Jun Zheng

LAB HEAD: Min ZHUANG

PROVIDER: PXD008715 | Pride | 2018-06-04

REPOSITORIES: Pride

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			Action	DRS
	CD28REP1.raw	Raw
	CD28REP2.raw	Raw
	CD28REP3.raw	Raw
	CONTROL1REP1.raw	Raw
	CONTROL1REP2.raw	Raw

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Publications

A proximity-tagging system to identify membrane protein-protein interactions.

Liu Qiang Q Zheng Jun J Sun Weiping W Huo Yinbo Y Zhang Liye L Hao Piliang P Wang Haopeng H Zhuang Min M

Nature methods 20180813 9

The communication between cells and between cellular organelles is often controlled by the interaction of membrane proteins. Although many methods for the detection of protein-protein interactions (PPIs) exist, membrane PPIs remain difficult to detect. Here we developed a proximity-based tagging system, PUP-IT (pupylation-based interaction tagging), to identify membrane protein interactions. In this approach, a small protein tag, Pup, is applied to proteins that interact with a PafA-fused bait, ...[more]

PMID: 30104635

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Project description:we have developed a new catalytic tagging system and have used it on the identification of interacting partners of membrane proteins

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