Dataset Information

Quantitative proteomics profiling determines the uniqueness of the sinus node

ABSTRACT: The sinus node is a collection of highly specialized cells that constitute the natural pacemaker activity of our heart. The protein expression landscape of the sinus node differs from the surrounding cardiac tissue, although it is primarily comprised of myocytes and fibroblasts like the rest of the cardiac tissue, endowing it with its unique ability to regulate heart rate. Here we performed quantitative proteomics experiments to profile protein expression in the pacemaker of the heart, and compared it to protein expression in the neighbouring atrial muscle. In summary, the quantitative proteomics data presented here offer a highly detailed insight into the unique composition of the pacemaker of our heart.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Nora Linscheid

LAB HEAD: Alicia Lundby

PROVIDER: PXD008736 | Pride | 2019-07-08

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	20160718_QE7_nLC3_PCP_Mouse_SinusNode_Technical_rep_1_SN1_Frac_01.raw	Raw
	20160718_QE7_nLC3_PCP_Mouse_SinusNode_Technical_rep_1_SN1_Frac_02.raw	Raw
	20160718_QE7_nLC3_PCP_Mouse_SinusNode_Technical_rep_1_SN1_Frac_03.raw	Raw
	20160718_QE7_nLC3_PCP_Mouse_SinusNode_Technical_rep_1_SN1_Frac_04.raw	Raw
	20160718_QE7_nLC3_PCP_Mouse_SinusNode_Technical_rep_1_SN1_Frac_05.raw	Raw

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Publications

Quantitative proteomics and single-nucleus transcriptomics of the sinus node elucidates the foundation of cardiac pacemaking.

Linscheid Nora N Logantha Sunil Jit R J SJRJ Poulsen Pi Camilla PC Zhang Shanzhuo S Schrölkamp Maren M Egerod Kristoffer Lihme KL Thompson Jonatan James JJ Kitmitto Ashraf A Galli Gina G Humphries Martin J MJ Zhang Henggui H Pers Tune H TH Olsen Jesper Velgaard JV Boyett Mark M Lundby Alicia A

Nature communications 20190628 1

The sinus node is a collection of highly specialised cells constituting the heart's pacemaker. The molecular underpinnings of its pacemaking abilities are debated. Using high-resolution mass spectrometry, we here quantify >7,000 proteins from sinus node and neighbouring atrial muscle. Abundances of 575 proteins differ between the two tissues. By performing single-nucleus RNA sequencing of sinus node biopsies, we attribute measured protein abundances to specific cell types. The data reveal signif ...[more]

PMID: 31253831

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