Dataset Information

Impact of CD74 knockdown on the HLA peptidome of the H1 melanoma cell line

ABSTRACT: The influence of CD74 expression on the HLA peptidome of the H1 melanoma cell line was analyzed by label-free quantitation mass spectrometry of HLA ligand extracts obtained from CD74 siRNA & control siRNA treated cells.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Melanocyte, Cell Culture

DISEASE(S): Melanoma

SUBMITTER: Daniel Kowalewski

LAB HEAD: Stefan Stevanovic

PROVIDER: PXD008937 | Pride | 2018-03-01

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	160121_DK_CoOp-PH_H1_CD74siRNA_Tue39L243_20_DoseFinding_msms4.msf	Msf
	160121_DK_CoOp-PH_H1_CD74siRNA_Tue39L243_20_DoseFinding_msms4.raw	Raw
	160121_DK_CoOp-PH_H1_CD74siRNA_W_20_DoseFinding_msms2.msf	Msf
	160121_DK_CoOp-PH_H1_CD74siRNA_W_20_DoseFinding_msms2.raw	Raw
	160121_DK_CoOp-PH_H1_CTRLsiRNA_Tue39L243_20_DoseFinding_msms3.msf	Msf

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Publications

CD74 regulates complexity of tumor cell HLA class II peptidome in brain metastasis and is a positive prognostic marker for patient survival.

Zeiner P S PS Zinke J J Kowalewski D J DJ Bernatz S S Tichy J J Ronellenfitsch M W MW Thorsen F F Berger A A Forster M T MT Muller A A Steinbach J P JP Beschorner R R Wischhusen J J Kvasnicka H M HM Plate K H KH Stefanović S S Weide B B Mittelbronn M M Harter P N PN

Acta neuropathologica communications 20180301 1

Despite multidisciplinary local and systemic therapeutic approaches, the prognosis for most patients with brain metastases is still dismal. The role of adaptive and innate anti-tumor response including the Human Leukocyte Antigen (HLA) machinery of antigen presentation is still unclear. We present data on the HLA class II-chaperone molecule CD74 in brain metastases and its impact on the HLA peptidome complexity.We analyzed CD74 and HLA class II expression on tumor cells in a subset of 236 human ...[more]

PMID: 29490700

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Project description:Biomaterials play an increasing role in clinical applications and regenerative medicine. Modern biomaterials should imitate the function of damaged tissue without triggering an immune response, initiate self-regeneration of the body and gradually degrade after implantation. The immune system is now well recognized to play a major role in influencing the biocompatibility of implanted medical devices. To develop a better understanding of the effects of biomaterials on the immune response, we have developed a highly sensitive novel test system capable of examining changes in the immune system by biomaterial. Here, we evaluate for the first time the immunopeptidome, a highly sensitive system that reflects cancer transformation, virus or drug influences and passes these changes directly to T cells, as a test system to examine the effects of contact with materials. Since monocytes are one of the first immune cells reacting to biomaterials, we have tested the influence of different materials - stainless steel, aluminum, zinc, high-density polyethylene, polyurethane films containing zinc diethyldithiocarbamate, copper, and zinc sulphate - on the monocytic THP-1 cell line. Material-associated peptides were identified after stimulation with all material types examined. The magnitude of induced changes in the immunopeptidome after the stimulation appears comparable to that of bacterial lipopolysaccharides (LPS). The source proteins of many detected peptides are associated with cytotoxicity, fibrosis, autoimmunity, inflammation and cellular stress. Considering all tested materials, it was found that the LPS-induced cytotoxicity-, inflammation- and cellular stress-associated HLA class I peptides were mainly induced by aluminum and HLA class II peptides mainly by stainless steel. These findings provide some of the first insights into the effects on the immunopeptidome by biomaterials. A more thorough understanding of these effects may enable the design of more biocompatible implant materials using in vitro models in future. This may be achieved through developing a deeper understanding of possible immune responses induced by biomaterials such as fibrosis, inflammation, cytotoxicity, and autoimmune reactions.