Dataset Information

The HLA-ligandome of oropharyngeal squamous cell carcinomas reveals shared tumor-exclusive peptides for semi-personalized vaccination

ABSTRACT: Oropharyngeal squamous cell carcinoma (OPSCC) is diagnosed in 93,000 patients worldwide per year and 51,000 annual deaths can be attributed to this disease. OPSCCs are either human papillomavirus associated (HPV-positive) cancers or non-virally induced, primarily tobacco and alcohol associated (HPV-negative) cancers. MS analysis enabled the identification of naturally HLA-presented peptides in OPSCC tumor tissue. By comparative profiling against benign HLA ligandomic datasets, we demonstrated that tumor-associated peptides are HLA-presented on the cell surfaces of OPSCCs. The established warehouse of OPSCC-associated peptides can be used for downstream immunogenicity testing and peptide-based immunotherapy in (semi-) personalized strategies.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Tongue, Tonsil, Palate

DISEASE(S): Head And Neck Squamous Cell Carcinoma

SUBMITTER: Lena Mühlenbruch

LAB HEAD: Simon Laban

PROVIDER: PXD033383 | Pride | 2023-02-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	OPSCC_01_ClassII_Rep-1.msf	Msf
	OPSCC_01_ClassII_Rep-1.raw	Raw
	OPSCC_01_ClassII_Rep-2.msf	Msf
	OPSCC_01_ClassII_Rep-2.raw	Raw
	OPSCC_01_ClassII_Rep-3.msf	Msf

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The HLA ligandome of oropharyngeal squamous cell carcinomas reveals shared tumour-exclusive peptides for semi-personalised vaccination.

Mühlenbruch Lena L Abou-Kors Tsima T Dubbelaar Marissa L ML Bichmann Leon L Kohlbacher Oliver O Bens Martin M Thomas Jaya J Ezić Jasmin J Kraus Johann M JM Kestler Hans A HA von Witzleben Adrian A Mytilineos Joannis J Fürst Daniel D Engelhardt Daphne D Doescher Johannes J Greve Jens J Schuler Patrick J PJ Theodoraki Marie-Nicole MN Brunner Cornelia C Hoffmann Thomas K TK Rammensee Hans-Georg HG Walz Juliane S JS Laban Simon S

British journal of cancer 20230223 9

<h4>Background</h4>The immune peptidome of OPSCC has not previously been studied. Cancer-antigen specific vaccination may improve clinical outcome and efficacy of immune checkpoint inhibitors such as PD1/PD-L1 antibodies.<h4>Methods</h4>Mapping of the OPSCC HLA ligandome was performed by mass spectrometry (MS) based analysis of naturally presented HLA ligands isolated from tumour tissue samples (n = 40) using immunoaffinity purification. The cohort included 22 HPV-positive (primarily HPV-16) and ...[more]

PMID: 36823366

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