Proteomics

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Proteomic analysis of S-nitrosylated nuclear proteins in rat cortical neurons

ABSTRACT: S-nitrosothiol Resin Assisted Capture(SNORAC) coupled with mass spectrometry analysis has been used to study S-nitrosylated proteins in rat cortical neurons. Proteins were captured on the resin by covalently attaching their former S-NO moiety. Then, nistrosylated proteins were eluted by on-bead digestion. Formerly S-nitrosylated peptides were also eluted from the beads in a second step, and analyzed separately for assessment of the nitrosylation site (qualitative analysis). Out of over 600 nitrosyltated proteins, 131 proteins have never been shown to be S-nitrosylated in any system and 612 are new targets of S-nitrosylation in cortical neurons. The site(s) of Snitrosylation were also identified for 59% of the targets.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Neuron Of Cerebral Cortex

SUBMITTER: Marco Gaspari  

LAB HEAD: Marco Gaspari

PROVIDER: PXD009071 | Pride | 2018-07-11

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
SNORAC-Exp1-2-peptides.msf Msf
SNORAC-Exp1-2-peptides.pep.xml Pepxml
SNORAC-Exp1-2-peptides.raw Raw
SNORAC-Exp1-A1L-A4H.msf Msf
SNORAC-Exp1-A1L-A4H.pep.xml Pepxml
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Publications

Proteomic analysis of S-nitrosylated nuclear proteins in rat cortical neurons.

Smith Jacob G JG   Aldous Sarah G SG   Andreassi Catia C   Cuda Giovanni G   Gaspari Marco M   Riccio Antonella A  

Science signaling 20180703 537

Neurons modulate gene expression in response to extrinsic signals to enable brain development, cognition, and learning and to process stimuli that regulate systemic physiological functions. This signal-to-gene communication is facilitated by posttranslational modifications such as S-nitrosylation, the covalent attachment of a nitric oxide (NO) moiety to cysteine thiols. In the cerebral cortex, S-nitrosylation of histone deacetylase 2 (HDAC2) is required for gene transcription during neuronal dev  ...[more]

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