Proteomics

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Stress-induced changes in the S-palmitoylation and S-nitrosylation of proteins involved in synaptic plasticity


ABSTRACT: Summary The precise regulation of synaptic integrity is critical for neuronal network connectivity and proper brain function. Essential aspects of the activity and localization of synaptic proteins are regulated by posttranslational modifications. S-palmitoylation is a reversible covalent modification of the cysteine with palmitate. It modulates affinity of the protein for cell membranes and membranous compartments. Intracellular palmitoylation dynamics are regulated by other posttranslational modifications, such as S-nitrosylation. Still unclear, however, are the ways in which this crosstalk is affected in brain pathology, such as stress-related disorders. Using a newly developed mass spectrometry-based approach (Palmitoylation And Nitrosylation Interplay Monitoring), we analyzed the endogenous S-palmitoylation and S-nitrosylation of postsynaptic density proteins at the level of specific single cysteines in a mouse model of chronic stress. Our results suggest that atypical mechanism of crosstalk between the S-palmitoylation and S-nitrosylation of synaptic proteins might be one of the major events associated with chronic stress disorders.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Monika Zareba-Koziol  

LAB HEAD: Jakub M Wlodarczyk

PROVIDER: PXD010822 | Pride | 2019-07-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
509292309zar_PAL_K1_s.dat Other
509292309zar_PAL_K1_s.raw Raw
509292310zar_PAL_K2_s.dat Other
509292310zar_PAL_K2_s.raw Raw
509292311zar_PAL_K3_s.dat Other
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Publications

Stress-induced Changes in the S-palmitoylation and S-nitrosylation of Synaptic Proteins.

Zareba-Koziol Monika M   Bartkowiak-Kaczmarek Anna A   Figiel Izabela I   Krzystyniak Adam A   Wojtowicz Tomasz T   Bijata Monika M   Wlodarczyk Jakub J  

Molecular & cellular proteomics : MCP 20190716 10


The precise regulation of synaptic integrity is critical for neuronal network connectivity and proper brain function. Essential aspects of the activity and localization of synaptic proteins are regulated by posttranslational modifications. S-palmitoylation is a reversible covalent modification of the cysteine with palmitate. It modulates affinity of the protein for cell membranes and membranous compartments. Intracellular palmitoylation dynamics are regulated by crosstalk with other posttranslat  ...[more]

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