Proteomics

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IsoGenie Abisko Stordalenmire LC-MSMS

ABSTRACT: Large amounts of carbon sequestered in permafrost are becoming available for microbial degradation. We investigated 1,529 microbial metagenome-assembled genomes recovered from our site to understand carbon processing in this environment. Metabolic reconstruction, supported by metatranscriptomic and metaproteomic data, revealed key populations involved in organic matter degradation, including bacteria encoding a pathway for xylose degradation only previously identified in fungi.

OTHER RELATED OMICS DATASETS IN: MODEL1109130000MODEL1311110000MODEL1909260003MODEL1311110001MODEL1909260004MODEL1703310000MODEL1909260005MODEL1909260006

INSTRUMENT(S): Q Exactive

ORGANISM(S): Uncultured Environmental Isolates Environmental Samples <archaea> Environmental Samples Eukaryota (eucaryotes)

SUBMITTER: Ahmed Zayed  

LAB HEAD: Virginia Isabel Rich

PROVIDER: PXD009096 | Pride | 2018-07-23

REPOSITORIES: Pride

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Action DRS
Iso_JGI_Aug_E2D_10_01_offline_27May17_Precious_16-11-43.mzML Mzml
Iso_JGI_Aug_E2D_10_01_offline_27May17_Precious_16-11-43.pride.mgf.gz Mgf
Iso_JGI_Aug_E2D_10_01_offline_27May17_Precious_16-11-43.raw Raw
Iso_JGI_Aug_E2D_10_02_offline_27May17_Precious_16-11-43.mzML Mzml
Iso_JGI_Aug_E2D_10_02_offline_27May17_Precious_16-11-43.pride.mgf.gz Mgf
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As global temperatures rise, large amounts of carbon sequestered in permafrost are becoming available for microbial degradation. Accurate prediction of carbon gas emissions from thawing permafrost is limited by our understanding of these microbial communities. Here we use metagenomic sequencing of 214 samples from a permafrost thaw gradient to recover 1,529 metagenome-assembled genomes, including many from phyla with poor genomic representation. These genomes reflect the diversity of this comple  ...[more]

PMID: 30013118

Publication: 1/2

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