Dataset Information

“Trading virulence forsurvival”:Metabolic pathway switch is a key mechanism for survival of Macrophomina phaseolina upon Burkholderia contaminans challenge

ABSTRACT: Macrophominaphaseolina is attributed to infect a wide range of plants. There are not a successful strategies to eradicate the fungus from the soil. In this study, a jute endophytic bacteria, Burkholderiacontaminans has been found to have a promising effect in controlling M. phaseolinain in vitro culture. Using the iTRAQ LC-MS/MS method for quantitative proteomics study, a comparative analysis of the whole proteome of M.phaseolinaunder both Burkholderiachallenged and unchallenged conditions were made and analyzed. A tough battle appears to ensue between the fungus and the bacterium, where Burkholderia manages to arrest the growth of the fungus and decrease its pathogenicity but, the latter apparently survives under ‘hibernating’ conditions by up-regulating its energy metabolism.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Macrophomina Phaseolina

TISSUE(S): Fungus

SUBMITTER: Bhoj Kumar

LAB HEAD: Tushar K. Maiti

PROVIDER: PXD009121 | Pride | 2020-02-18

REPOSITORIES: Pride

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Proteome Analyses Reveal Macrophomina phaseolina's Survival Tools When Challenged by Burkholderia contaminans NZ.

Zaman Nazia R NR Kumar Bhoj B Nasrin Zulia Z Islam Mohammad R MR Maiti Tushar K TK Khan Haseena H

ACS omega 20200115 3

A phytopathogenic fungus, Macrophomina phaseolina, which infects a wide range of plants, is an important consideration in agronomy. A jute endophytic bacterium, Burkholderia contaminans NZ, was found to have a promising effect in controlling the fungus in in vitro culture conditions. Using the iTRAQ LC-MS/MS method for quantitative proteomics study, an analysis of the whole proteome of Macrophomina phaseolina with or without B. contaminans NZ challenge identified 2204 ...[more]

PMID: 32010805

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Project description:Filamentous fungi are powerful producers of hydrolytic enzymes for the deconstruction of plant cell wall polysaccharides. However, the central question of how these sugars are perceived in the context of the complex cell wall matrix remains largely elusive. To address this question in a systematic fashion we performed an extensive comparative systems analysis of how the model filamentous fungus Neurospora crassa responds to the three main cell wall polysaccharides: pectin, hemicellulose and cellulose. We found the pectic response to be largely independent of the cellulolytic one with some overlap to hemicellulose, and in its extent surprisingly high, suggesting advantages for the fungus beyond being a mere carbon source. Our approach furthermore allowed us to identify carbon source-specific adaptations, such as the induction of the unfolded protein response on cellulose, and a commonly induced set of 29 genes likely involved in carbon scouting. Moreover, by hierarchical clustering we generated a co-expression matrix useful for the discovery of new components involved in polysaccharide utilization. This is exemplified by the identification of lat-1, which we demonstrate to encode for the physiologically relevant arabinose transporter in Neurospora. The analyses presented here are an important step towards understanding fungal degradation processes of complex biomass. Our data are based on carbon source transfer experiments. For this, N. crassa pregrown in sucrose for 16 hrs was washed in media without carbon and then transferred to either pectin, orange peel powder (OPP), xylan, Avicel (cellulose), sucrose, or media without carbon (NoC) for another 4 hrs. Biological triplicates (pectin, Avicel, sucrose, NoC) or duplicates (OPP) were used to identify differentially expressed genes in WT. For xylan only a single library was prepared, which was found to correlate well with previously published microarray data (Sun et al. 2012; doi: 10.1128/EC.05327-11)

			Action	DRS
	MACPH_10_1.wiff	Wiff
	MACPH_10_1.wiff.scan	Wiff
	MACPH_10_2.wiff	Wiff
	MACPH_10_2.wiff.scan	Wiff
	MACPH_11_1.wiff	Wiff

Dataset Information

“Trading virulence forsurvival”:Metabolic pathway switch is a key mechanism for survival of Macrophomina phaseolina upon Burkholderia contaminans challenge

Dataset's files

Publications

Proteome Analyses Reveal <i>Macrophomina phaseolina</i>'s Survival Tools When Challenged by <i>Burkholderia contaminans</i> NZ.

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