Dataset Information

Modern Paint Binder Controls for Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

ABSTRACT: Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and mallard egg white. Analysis of posttranslational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV-light and was likely applied long before the addition of the glue. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (bovine) Gallus Gallus (chicken)

TISSUE(S): Egg, Skin, Bone, Tendon

SUBMITTER: Meaghan Mackie

LAB HEAD: Enrico Cappellini

PROVIDER: PXD009298 | Pride | 2018-04-05

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20180215_QE3_nLC11_MEM_COLLAB_GeoG_1295_LaboratoryBlank.raw	Raw
	20180215_QE3_nLC11_MEM_COLLAB_GeoG_1299.raw	Raw
	20180215_QE3_nLC11_MEM_COLLAB_GeoG_1300.raw	Raw
	20180215_QE3_nLC11_MEM_COLLAB_GeoG_1303.raw	Raw
	20180219_QE3_nLC11_MEM_COLLAB_GeoG_1303_2.raw	Raw

Items per page:

1 - 5 of 8

Publications

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting.

Mackie Meaghan M Rüther Patrick P Samodova Diana D Di Gianvincenzo Fabiana F Granzotto Clara C Lyon David D Peggie David A DA Howard Helen H Harrison Lynne L Jensen Lars Juhl LJ Olsen Jesper V JV Cappellini Enrico E

Angewandte Chemie (International ed. in English) 20180426 25

Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post-translational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV l ...[more]

PMID: 29603563

Similar Datasets

Project description:Diagnosis of acute infection in the critically ill remains a challenge. Changes in physiologic parameters and existing molecular diagnostics are not specific and microbiologic confirmation of infection can take days. As cellular first responders, preclinical studies indicate that circulating leukocytes are activated in response to bacterial infection, manifesting infection-specific transcriptional programs. We hypothesized that circulating leukocyte transcriptional profiles can be used to diagnose infection and monitor response to therapy in the critically ill. Experiment Overall Design: A translational research approach was employed. Fifteen mice underwent intratracheal (i.t.) injections of live P. aeruginosa, P. aeruginosa endotoxin, live S. pneumoniae, or normal saline. At 24 hours after injury, GeneChip microarray analysis of circulating buffy coat RNA identified 219 genes that distinguished between the pulmonary insults and differences in 7-day mortality. Similarly, buffy coat microarray expression profiles were generated from 20 mechanically ventilated patients every two days for up to three weeks; 11 of these patients developed pneumonia. Principal components analysis of the expression levels of the human orthologs for the mouse genes resulted in gene expression trajectories that describe the cohort of 11 patients as they develop, are treated for, and convalesce from ventilator-associated pneumonia (VAP). When the VAP microarray profiles were analyzed independently, 85 genes were identified with consistent changes in abundance during the seven days bracketing the diagnosis of VAP. The estimated prediction accuracy in this small patient cohort was 91%. The abundance of select transcripts was validated using real-time PCR. Principal components analysis of the average microarray-measured abundances of these genes defined a general trajectory (riboleukogram) for the onset of acute infection in this patient population. Individual riboleukograms demonstrated that the onset and course of infection are specific for each individual, but as patients recover from infection the riboleukograms converge. The gene ontologies of the co-expression networks indicate that leukocyte chemokines and granule maturation are especially informative for pneumonia diagnostics.