Proteomics

Dataset Information

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The FAM83 family of proteins are anchors for Casein Kinase 1 isoforms through the DUF1669 domain

ABSTRACT: The Casein Kinase 1 (CK1) family of Ser/Thr protein kinases are implicated in the regulation of many cellular processes, including cell cycle, circadian rhythm, Wnt and Sonic Hedgehog signalling. Regarded as constitutively active kinases, their regulation in cells is critically important but poorly understood. We report here that members of the FAM83 family of uncharacterized proteins are central regulators of CK1 isoforms in cells. The eight members of the FAM83 family (A-H) interact and co-localize with different CK1 alpha, alpha-like, delta and epsilon isoforms. We demonstrate that the interaction with CK1 isoforms is mediated through a number of residues within a conserved domain of unknown function, termed DUF1669, which characterises the FAM83 family. CK1-binding deficient mutants of FAM83 proteins interfere with the subcellular localization of CK1 isoforms as well as FAM83 proteins themselves and their cellular functions. We propose that DUF1669 be renamed the Polypeptide Anchor of CK1 (PACK1) domain.

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Gopal Sapkota  

LAB HEAD: Gopal P Sapkota

PROVIDER: PXD009335 | Pride | 2018-05-24

REPOSITORIES: Pride

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F199990.xml Xml
F199991.xml Xml
F199992.xml Xml
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Publications

Characterisation of the biochemical and cellular roles of native and pathogenic amelogenesis imperfecta mutants of FAM83H.

Tachie-Menson Theresa T   Gázquez-Gutiérrez Ana A   Fulcher Luke J LJ   Macartney Thomas J TJ   Wood Nicola T NT   Varghese Joby J   Gourlay Robert R   Soares Renata F RF   Sapkota Gopal P GP  

Cellular signalling 20200411

The majority of mutations identified in patients with amelogenesis imperfecta have been mapped to FAM83H. As FAM83H expression is not limited to the enamel, how FAM83H contributes to amelogenesis is still largely unknown. We previously reported that members of the FAM83 family of proteins interact with and regulate the subcellular distribution of the promiscuous serine-threonine protein kinase CK1 family, through their shared N-terminal DUF1669 domains. FAM83H co-localises with CK1 isoforms to s  ...[more]

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