SILAC APMS analysis of CCDC103 interactome
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ABSTRACT: To get insights into the function of CCD103, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-CCDC103 and control cells with isotopically labelled amino-acids (SILAC), whole cell extracts were immuno-precipitated (IP) with anti-GFP antibodies and immunoprecipitates were subjected to quantitative mass-spectrometry analysis. SILAC labels: K0R0 control Hela H9 vs K4R6 x-FLAG- GFP-CCDC103
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Hela Cell
SUBMITTER: Franck Vandermoere
LAB HEAD: Edouard Bertrand
PROVIDER: PXD009498 | Pride | 2018-06-08
REPOSITORIES: Pride
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