Proteomics

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SILAC APMS analysis of CCDC103 interactome

ABSTRACT: To get insights into the function of CCD103, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-CCDC103 and control cells with isotopically labelled amino-acids (SILAC), whole cell extracts were immuno-precipitated (IP) with anti-GFP antibodies and immunoprecipitates were subjected to quantitative mass-spectrometry analysis. SILAC labels: K0R0 control Hela H9 vs K4R6 x-FLAG- GFP-CCDC103

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Franck Vandermoere  

LAB HEAD: Edouard Bertrand

PROVIDER: PXD009498 | Pride | 2018-06-08

REPOSITORIES: Pride

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Publications

EEG and behavior reactions upon stimulation of nonspecific thalamic nuclei in unanesthetized cats.

Roitbak A I AI   Eristavi N N  

Acta biologiae experimentalis 19660101 4

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