Proteomics

Dataset Information

0

SILAC APMS analysis of Pih1D2 interactome

ABSTRACT: To get insights into the function of Pih1D2 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-Pih1D2 and control cells with isotopically labelled amino-acids (SILAC), whole cell extracts were immuno-precipitated (IP) with anti-GFP antibodies and immunoprecipitates were subjected to quantitative mass-spectrometry analysis. SILAC labels: K0R0 control Hela H9 vs K4R6 x-FLAG- GFP- Pih1D2

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Franck Vandermoere  

LAB HEAD: Edouard Bertrand

PROVIDER: PXD009520 | Pride | 2018-06-10

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
CPS_human_SPTr_canonical_150114.fasta Fasta
PHAXRNASE_170301_1_E130314_B01.raw Raw
PHAXRNASE_170301_1_E130314_B02.raw Raw
PHAXRNASE_170301_1_E130314_B03.raw Raw
PHAXRNASE_170301_1_E130314_B04.raw Raw
Items per page:
1 - 5 of 18
altmetric image

Publications

R2TP is an HSP90 co-chaperone that assembles important macro-molecular machineries. It is composed of an RPAP3-PIH1D1 heterodimer, which binds the two essential AAA+ATPases RUVBL1/RUVBL2. Here, we resolve the structure of the conserved C-terminal domain of RPAP3, and we show that it directly binds RUVBL1/RUVBL2 hexamers. The human genome encodes two other proteins bearing RPAP3-C-terminal-like domains and three containing PIH-like domains. Systematic interaction analyses show that one RPAP3-like  ...[more]

Similar Datasets

Proteomics SILAC APMS analysis of RPAP3 C-term interactome
2018-06-10 | PXD009518 | Pride
Proteomics SILAC APMS analysis of CCDC103 interactome
2018-06-08 | PXD009498 | Pride
Proteomics SILAC APMS analysis of DNAAF2 interactome
2018-06-10 | PXD009501 | Pride
Proteomics SILAC APMS analysis of Pih1D3 interactome
2018-06-10 | PXD009499 | Pride
Proteomics SILAC APMS analysis of SRP19 interactome in HEK-293 cells
2024-06-07 | PXD042195 | Pride
Proteomics Interactome of NOP58 WT vs KA_AR mutant
2020-12-14 | PXD019415 | Pride
Proteomics Interactome of Prpf31 Wild-type and A216P
2020-12-14 | PXD019390 | Pride
Proteomics SILAC APMS analysis of NHP2 / Gar1 interactome in HEK-293 cells
2023-05-10 | PXD026749 | Pride
Proteomics Interactome of Prpf31 WT vs KA_AR mutant
2020-12-14 | PXD019416 | Pride
Proteomics Interactome of HNTG c12orf45 protein
2020-12-14 | PXD019389 | Pride