Dataset Information

SILAC APMS analysis of Pih1D3 interactome

ABSTRACT: To get insights into the function of Pih1D3 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-Pih1D3 and control cells with isotopically labelled amino-acids (SILAC), whole cell extracts were immuno-precipitated (IP) with anti-GFP antibodies and immunoprecipitates were subjected to quantitative mass-spectrometry analysis. SILAC labels: K0R0 control Hela H9 vs K4R6 x-FLAG- GFP- Pih1D3

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Franck Vandermoere

LAB HEAD: Edouard Bertrand

PROVIDER: PXD009499 | Pride | 2018-06-10

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	MaxQuantOutput.tar.gz	Other
	PHAXRNASE_160425_1_E160510_01.raw	Raw
	PHAXRNASE_160425_1_E160510_02.raw	Raw
	PHAXRNASE_160425_1_E160510_03.raw	Raw
	PHAXRNASE_160425_1_E160510_04.raw	Raw

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Publications

The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones.

Maurizy Chloé C Quinternet Marc M Abel Yoann Y Verheggen Céline C Santo Paulo E PE Bourguet Maxime M C F Paiva Ana A Bragantini Benoît B Chagot Marie-Eve ME Robert Marie-Cécile MC Abeza Claire C Fabre Philippe P Fort Philippe P Vandermoere Franck F M F Sousa Pedro P Rain Jean-Christophe JC Charpentier Bruno B Cianférani Sarah S Bandeiras Tiago M TM Pradet-Balade Bérengère B Manival Xavier X Bertrand Edouard E

Nature communications 20180529 1

R2TP is an HSP90 co-chaperone that assembles important macro-molecular machineries. It is composed of an RPAP3-PIH1D1 heterodimer, which binds the two essential AAA+ATPases RUVBL1/RUVBL2. Here, we resolve the structure of the conserved C-terminal domain of RPAP3, and we show that it directly binds RUVBL1/RUVBL2 hexamers. The human genome encodes two other proteins bearing RPAP3-C-terminal-like domains and three containing PIH-like domains. Systematic interaction analyses show that one RPAP3-like ...[more]

PMID: 29844425

Dataset Information

SILAC APMS analysis of Pih1D3 interactome

Dataset's files

Publications

The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones.

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