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Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small Molecules

ABSTRACT: The protein corona, a dynamic biomolecular layer that forms on nanoparticle (NP) surfaces upon exposure to biological fluids is emerging as a valuable diagnostic tool for improving plasma proteome coverage analyzed by liquid chromatography-mass spectrometry (LC-MS/MS). Here, we show that spiking phosphatidylcholine into plasma can induce diverse protein corona patterns on otherwise identical NPs, significantly enhancing the depth of plasma proteome profiling. This significant achievement is made utilizing only a single NP type and one small molecule to analyze a single plasma sample, setting a new standard in proteomic depth of the plasma sample. Given the critical role of plasma proteomics in biomarker discovery and disease monitoring, we anticipate widespread adoption of this methodology for identification and clinical translation of proteomic biomarkers into FDA approved diagnostics.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Liangliang Sun

LAB HEAD: Liangliang Sun

PROVIDER: PXD053359 | Pride | 2024-10-15

REPOSITORIES: Pride

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			Action	DRS
	05292024_S2_1ul.tsv	Tabular
	05292024_S2_1ul_.raw	Raw
	05292024_S2_1ul_2.raw	Raw
	05292024_S2_1ul_2.tsv	Tabular
	05292024_S2_1ul_3.raw	Raw

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