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SPIO corona - Immunoglobulin deposition on biomolecule corona determines complement opsonization efficiency of preclinical and clinical nanoparticles

ABSTRACT: Identification of corona proteins in serum and plasma of healthy donors

INSTRUMENT(S): impact HD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma, Blood Serum

SUBMITTER: Dmitri Simberg

LAB HEAD: Dmitri Simberg

PROVIDER: PXD011781 | Pride | 2019-01-14

REPOSITORIES: Pride

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			Action	DRS
	F22.dat	Other
	F22plasma.baf	Other
	F38.dat	Other
	F38plasma.baf	Other
	F44.dat	Other

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Publications

Immunoglobulin deposition on biomolecule corona determines complement opsonization efficiency of preclinical and clinical nanoparticles.

Vu Vivian P VP Gifford Geoffrey B GB Chen Fangfang F Benasutti Halli H Wang Guankui G Groman Ernest V EV Scheinman Robert R Saba Laura L Moghimi Seyed Moein SM Simberg Dmitri D

Nature nanotechnology 20190114 3

Deposition of complement factors (opsonization) on nanoparticles may promote clearance from the blood by macrophages and trigger proinflammatory responses, but the mechanisms regulating the efficiency of complement activation are poorly understood. We previously demonstrated that opsonization of superparamagnetic iron oxide (SPIO) nanoworms with the third complement protein (C3) was dependent on the biomolecule corona of the nanoparticles. Here we show that natural antibodies play a critical rol ...[more]

PMID: 30643271

Similar Datasets

Immunoglobulin deposition on biomolecule corona determines complement opsonization efficiency of preclinical and clinical nanoparticles.

Project description:Deposition of complement factors (opsonization) on nanoparticles may promote clearance from the blood by macrophages and trigger proinflammatory responses, but the mechanisms regulating the efficiency of complement activation are poorly understood. We previously demonstrated that opsonization of superparamagnetic iron oxide (SPIO) nanoworms with the third complement protein (C3) was dependent on the biomolecule corona of the nanoparticles. Here we show that natural antibodies play a critical role in C3 opsonization of SPIO nanoworms and a range of clinically approved nanopharmaceuticals. The dependency of C3 opsonization on immunoglobulin binding is almost universal and is observed regardless of the complement activation pathway. Only a few surface-bound immunoglobulin molecules are needed to trigger complement activation and opsonization. Although the total amount of plasma proteins adsorbed on nanoparticles does not determine C3 deposition efficiency, the biomolecule corona per se enhances immunoglobulin binding to all nanoparticle types. We therefore show that natural antibodies represent a link between biomolecule corona and C3 opsonization, and may determine individual complement responses to nanomedicines.

| S-EPMC6402998 | biostudies-literature

Pan-cancer proteomic map of 949 human cell lines (ProCan-DepMapSanger)

Project description:This pan-cancer cell line proteomic atlas comprises proteomic data acquired by data independent acquisition (specifically, SWATH) mass spectrometry for 949 cancer cell lines. Cell lines were processed in technical triplicate, with duplicates acquired on different mass spectrometers, alongside HEK293T cell line control samples processed across the experimental period. For further details, refer to the publication that accompanies this data deposition.

2022-07-13 | PXD030304 | Pride

Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small Molecules

Project description:The protein corona, a dynamic biomolecular layer that forms on nanoparticle (NP) surfaces upon exposure to biological fluids is emerging as a valuable diagnostic tool for improving plasma proteome coverage analyzed by liquid chromatography-mass spectrometry (LC-MS/MS). Here, we show that spiking phosphatidylcholine into plasma can induce diverse protein corona patterns on otherwise identical NPs, significantly enhancing the depth of plasma proteome profiling. This significant achievement is made utilizing only a single NP type and one small molecule to analyze a single plasma sample, setting a new standard in proteomic depth of the plasma sample. Given the critical role of plasma proteomics in biomarker discovery and disease monitoring, we anticipate widespread adoption of this methodology for identification and clinical translation of proteomic biomarkers into FDA approved diagnostics.

2024-10-15 | PXD053359 | Pride

Mass spectrometry-based top-down proteomics in nanomedicine: proteoform-specific measurement of protein corona

Project description:Conventional mass spectrometry (MS)-based bottom-up proteomics (BUP) analysis of protein corona [i.e., an evolving layer of biomolecules, mostly proteins, formed on the surface of nanoparticles (NPs) during their interactions with biomolecular fluids] enabled nanomedicine community to partly identify the biological identity of NPs. Such an approach, however, fails pinpoint the specific proteoforms—distinct molecular variants of proteins, which is essential for prediction of the biological fate and pharmacokinetics of nanomedicines. Recognizing this limitation, this study pioneers a robust and reproducible MS-based top-down proteomics (TDP) technique for precisely characterizing proteoforms in the protein corona. Our TDP approach has successfully identified hundreds of proteoforms in the protein corona of polystyrene NPs, ranging from 3-70 kDa, revealing over 20 protein biomarkers with combinations of post-translational modifications, signal peptide cleavages, and/or truncations—details that BUP could not fully discern. This advancement in MS-based TDP offers a more comprehensive and exact characterization of NP protein coronas, deepening our understanding of NPs' biological identities and potentially revolutionizing the field of nanomedicine.

2024-10-15 | PXD050779 | Pride

In situ Analysis of Nanoparticle Soft Corona and Dynamic Evolution

Project description:How soft corona i.e., the protein corona’s outer layer contributes to “biological” identity of nanomaterials (NMs) is largely unknown due to a longstanding challenge in capturing protein composition of the soft corona in situ. We herein develop an in situ “Fishing” method that can monitor the dynamic formation of protein corona on ultra-small chiral Cu2S nanoparticles (NPs) allowing us to directly separate and identify their protein composition. This method can detect spatiotemporal processes in the evolution of hard and soft coronas on chiral NPs, revealing subtle differences in their composition even within several minutes. This study highlights the importance of in situ and dynamic analysis of soft/hard corona composition, provides detailed insights into the roles of protein corona composition in mediating the biological responses of NPs, and offers a universal strategy to characterize the soft corona that will serve as a powerful tool for rational design of biomedical NMs.

2022-10-13 | PXD033976 | Pride

Protein Corona of 60nm Silver Nanoparticles

Project description:We studied the composition of the protein corona formed on 60 nm silver nanoparticles with citrate coating interacted with the human blood plasma under various pH and temperature conditions. The protein corona was analyzed using LC-MS and label-free quantitation.

2019-01-23 | PXD007648 | Pride

Plasma protein corona on superparamagnetic iron oxide nanoparticles (SPIONs)

Project description:We determined the composition of the plasma protein corona on silica-coated versus dextran-coated iron oxide nanoparticles using mass spectrometry-based proteomics approaches. Gene ontology (GO) enrichment analysis and Ingenuity Pathway Analysis (IPA) revealed distinct protein corona compositions for the two different SPIONs. Relaxivity of silica-coated SPIONs was modulated by the presence of a protein corona. Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.

2015-10-12 | PXD000766 | Pride

Protein corona formation on nanoplastics: polystyrene and PVC

Project description:In this study, the proteins forming the protein corona on PS and PVC were investigated after 5 min, 1 h, 6 h, or 24 h incubation of the nanoplastics with human plasma. TMT-based untargeted proteomics were applied to identify the proteins forming the protein corona.

2024-10-25 | PXD032162 | Pride

Analysis of plasma protein corona of liposomal formulations

Project description:Nanoparticles interact with biological fluids and plasma proteins at nano-bio interface affect their biopharmaceutical properties and biodistribution due to protein corona composition. The PrC composition of various designed LFs was evaluated ex vivo in human plasma by label-free quantitative proteomics.

2024-09-05 | PXD052701 | Pride

Plasma Protein Corona on Polystyrene Nanoparticles Facilitates their Transfer across the Human Placenta

Project description:Nanoparticles exposed to biological fluids are rapidly surrounded by proteins. It is known that this formed protein corona influences the interplay of nanoparticles with cells or tissue barriers. In this study, we report the impact of a formed human plasma protein corona on the transfer of 80 nm polystyrene (PS) nanoparticles across the human placenta. We used the human ex-vivo placental perfusion model, as it reflects the intact and physiological tissue barrier between mother and unborn. Our results show an enhanced transfer of polystyrenes, exposed to human plasma, across the placenta compared to bovine serum albumin which served as control setting. We isolated nanoparticles before and after tissue exposure and analyzed their protein corona via shotgun proteomics and LC-MS/MS. The corona profile of particles that crossed the placenta highlighted several proteins as possible drivers for elevated tissue transfer. Subsequently two distinct proteins, human albumin and immunoglobulin G, were selected and incubated with the nanoparticles to form a sole protein corona. Strikingly, the protein corona formed by albumin induced significantly the transfer of polystyrenes across the tissue as compared to corona formed by immunoglobulins. To conclude, our study provides a comparative analysis between different formed protein coronas on nanoparticles and a corona dependent transfer behavior of polystyrenes across placental tissue. Our findings suggest that protein corona analyses of nanoparticles might help to understand better their properties at biological barriers.

2020-08-19 | PXD018160 | Pride

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