Dataset Information

GlyXtoolMS Example Data Sets - glyXtoolMS: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data

ABSTRACT: The data sets of human IgG and human fibrinogen are used as test data for the publication of glyXtoolMS (https://github.com/glyXera/glyXtoolMS). GlyXtoolMS is an open-source analysis software for the (semi)automated targeted analysis of glycopeptide mass spectrometry data using OpenMS/TOPPAS as a framework and pipeline engine. The proteins were selected to show the successful analysis of both N-glycopeptide and O-glycopeptide samples. The samples were measured by nano reversed phase liquid chromatography coupled online to an electrospray ionization orbitrap mass spectrometer (nano RP-LC ESI- OT-MS/MS; LTQ Orbitrap Elite, Thermo Scientific, Waltham, MA, USA) with HCD fragmentation.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Markus Pioch

LAB HEAD: Erdmann Rapp

PROVIDER: PXD009716 | Pride | 2018-09-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20160417_MH_Fib_FASP_Tryp_HILIC_Enri_HCDstep.raw	Raw
	20160417_MH_IgG_FASP_Tryp_HILIC_Enri_HCDstep.raw	Raw
	MSData.zip	Other

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Publications

glyXtool<sup>MS</sup>: An Open-Source Pipeline for Semiautomated Analysis of Glycopeptide Mass Spectrometry Data.

Pioch Markus M Hoffmann Marcus M Pralow Alexander A Reichl Udo U Rapp Erdmann E

Analytical chemistry 20180925 20

For glycoproteomic analyses several web tools and standalone software packages have been developed over the recent years. These tools support or replace the time-consuming, cumbersome, and error-prone manual spectra analysis and glycopeptide identification. However, existing software tools are usually tailored to one fragmentation technique and only present the final analysis results. This makes manual inspection and correction of intermediate results difficult or even impossible. We solved this ...[more]

PMID: 30252445

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