Dataset Information

Mitochondrial Network Organization during Neuronal Reprogramming, MT_DF_SEC

ABSTRACT: The study focuses on an extensive biochemical fractionation with in-depth quantitative mass spectrometric profiling in the mitochondrial (mt) extracts of cultured human NTera2 embryonal carcinoma stem cells (i.e. ECSCs or undifferentiated state) and upon exposure to retinoic acid-induced differentiated neurons (DNs) to establish a network of high-quality mt protein-protein interactions. The resulting network showed that most of the native mt protein complexes with predicted subunits are previously unreported and endured extensive changes during neuronal differentiation and influence neuronal function and neurodegenerative disorder attributes.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Sadhna Phanse

LAB HEAD: Mohan Babu

PROVIDER: PXD009833 | Pride | 2020-01-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	FINV_Hs_9606_SP_170721.fasta	Fasta
	ZM170323_OT_BM0273-20_FR-1-1_NOTAG_TRITON_UREA_NTERA2-HUMAN.mzXML	Mzxml
	ZM170323_OT_BM0273-20_FR-1-1_NOTAG_TRITON_UREA_NTERA2-HUMAN.mzXML.mzid	Mzxml
	ZM170323_OT_BM0273-20_FR-1-1_NOTAG_TRITON_UREA_NTERA2-HUMAN.mzXML_dta.tar.bz2	Mzxml
	ZM170323_OT_BM0273-20_FR-1-1_NOTAG_TRITON_UREA_NTERA2-HUMAN.mzXML_out.tar.bz2	Mzxml

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Publications

Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis.

Moutaoufik Mohamed Taha MT Malty Ramy R Amin Shahreen S Zhang Qingzhou Q Phanse Sadhna S Gagarinova Alla A Zilocchi Mara M Hoell Larissa L Minic Zoran Z Gagarinova Maria M Aoki Hiroyuki H Stockwell Jocelyn J Jessulat Matthew M Goebels Florian F Broderick Kirsten K Scott Nichollas E NE Vlasblom James J Musso Gabriel G Prasad Bhanu B Lamantea Eleonora E Garavaglia Barbara B Rajput Alex A Murayama Kei K Okazaki Yasushi Y Foster Leonard J LJ Bader Gary D GD Cayabyab Francisco S FS Babu Mohan M

iScience 20190904

Mitochondrial protein (MP) assemblies undergo alterations during neurogenesis, a complex process vital in brain homeostasis and disease. Yet which MP assemblies remodel during differentiation remains unclear. Here, using mass spectrometry-based co-fractionation profiles and phosphoproteomics, we generated mitochondrial interaction maps of human pluripotent embryonal carcinoma stem cells and differentiated neuronal-like cells, which presented as two discrete cell populations by single-cell RNA se ...[more]

PMID: 31536960

Dataset Information

Mitochondrial Network Organization during Neuronal Reprogramming, MT_DF_SEC

Dataset's files

Publications

Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis.

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