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Impacts of HLA-B57 micropolymorphism (MRM)

ABSTRACT: Comparison of peptide ligands of major histocompatibility class (MHC) I isolated by immunoaffinity purification from the C1R (Class I reduced) B-lymphoblastoid cell line, transfected with the MHC class I alleles HLA-B*57:01, HLA-B*57:03 and HLA-B*58:01.

INSTRUMENT(S): Q TRAP

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Patricia Illing

LAB HEAD: Anthony W. Purcell

PROVIDER: PXD009850 | Pride | 2018-10-24

REPOSITORIES: Pride

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			Action	DRS
	B5701_rep1.wiff	Wiff
	B5701_rep1.wiff.scan	Wiff
	B5701_rep2.wiff	Wiff
	B5701_rep2.wiff.scan	Wiff
	B5701_rep3.wiff	Wiff

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Publications

HLA-B57 micropolymorphism defines the sequence and conformational breadth of the immunopeptidome.

Illing Patricia T PT Pymm Phillip P Croft Nathan P NP Hilton Hugo G HG Jojic Vladimir V Han Alex S AS Mendoza Juan L JL Mifsud Nicole A NA Dudek Nadine L NL McCluskey James J Parham Peter P Rossjohn Jamie J Vivian Julian P JP Purcell Anthony W AW

Nature communications 20181108 1

Immunophenotypic differences between closely related human leukocyte antigen (HLA) alleles have been associated with divergent clinical outcomes in infection, autoimmunity, transplantation and drug hypersensitivity. Here we explore the impact of micropolymorphism on peptide antigen presentation by three closely related HLA molecules, HLA-B*57:01, HLA-B*57:03 and HLA-B*58:01, that are differentially associated with the HIV elite controller phenotype and adverse drug reactions. For each allotype, ...[more]

PMID: 30410026

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Kinetics of abacavir-induced remodelling of the Major Histocompatibility Complex class I peptide repertoire

Project description:Characterisation of the kinetics of abacavir-induced perturbation of the immunopeptidome of the Major Histocompatibility Complex (MHC) class I molecule HLA-B*57:01.

2021-05-11 | PXD024331 | Pride

Impact of abacavir and abacavir analogues on HLA-B*57:01 peptide presentation

Project description:Characterisation of peptide ligands of the Major histocompatibility class (MHC) I allele, HLA-B*57:01, expressed in the C1R (Class I reduced) B-lymphoblastoid cell line, after exposure to the anti-retroviral abacavir or abacavir analogues.

2019-10-03 | PXD015398 | Pride

Direct quantification of viral epitope abundance reveals the importance of direct and cross-presentation in driving CTL responses during influenza infection

Project description:The magnitude of CD8+ T lymphocyte (CTL) responses to infection is a function of the available naïve T cell repertoire, combined with the context and duration of antigen presentation. Whilst T cell repertoires are relatively easily studied, the context and abundance of epitopes presented on infected cells and dendritic cells (DCs) and their subsequent impact on CTL responses are generally poorly understood. Using quantitative mass spectrometry, we identified and quantified the abundance of 21 class 1 Major Histocompatibility Complex molecule (MHCI)-restricted influenza A virus (IAV)-derived peptides following either direct presentation or cross-presentation. All identified peptides, including seven novel epitopes, elicited T cell responses in infected C57BL/6 mice. Quantitation of IAV epitopes displayed via direct presentation showed a maintenance of relative epitope abundance across distinct cell types, reflecting common antigen processing mechanisms. Comparison of epitope levels displayed via direct presentation and cross-presentation revealed a broad range of directly presented epitope abundances, which was normalised during cross-presentation. Further, we observed a clear disparity in the abundance of the two key immunodominant IAV antigens, wherein direct infection drove optimal nucleoprotein (NP)366-374 presentation, while cross-presentation was optimal for acid polymerase (PA)224-233 presentation. This study provides a detailed dissection of viral pMHCI abundance after infection and reveals the importance of both direct and cross-presentation in driving dominant CTL responses. The study also demonstrates how empirical assessment of epitope abundance in both modes of antigen presentation is necessary to fully understand the immunogenicity and response magnitude to T cell epitopes.

2019-07-02 | PXD012728 | Pride

LFQ data for: Direct quantification of viral epitope abundance reveals the importance of direct and cross-presentation in driving CTL responses during influenza infection

2019-07-02 | PXD012776 | Pride

Analysis of human leukocyte antigen (HLA) allotype-specific response in abacavir-exposed keratinocyte

Project description:Abacavir, a nucleoside reverse-transcriptase inhibitor used for the treatment of human immunodeficiency virus (HIV) infection, develops hypersensitivity in patients carrying HLA-B*57:01 allele through drug antigen presentation. We found that abacavir exposure induced HLA allotype-specific innate immune response in keratinocyte derived from HLA-B*57:01 transgenic mice. Therefore, the mechanism of the novel response was analyzed comprehensively.

2018-03-01 | GSE110394 | GEO

C1R.B*57:01 MHC class I immunopeptidome LC-MSMS

2018-10-24 | PXD008570 | Pride

C1R.A*01:01, C1R.A*02:01, C1R.A*24:02 MHC class I immunopeptidome LC-MSMS

Project description:Characterisation of peptide ligands of Major histocompatibility class (MHC) I isolated by immunoaffinity purification from the C1R (Class I reduced) B-lymphoblastoid cell line, transfected with the MHC class I allele HLA-A*01:01, or HLA-A*02:01, HLA-A*24:02. In addition, public mass spectrometry (MS) datasets of HLA-I and HLA-II immunopeptidome derived from patients’ samples, PBMC or cell lines, and shotgun proteomics from trypsin/elastase digestion were analysed.

2020-07-27 | PXD014754 | Pride

Influenza-derived peptide presentation by HLA-A*11:01

Project description:Analysis of the immunopeptidome of Human Leukocyte Antigen (HLA)-A*11:01 during influenza infection. Analyses were performed using the Class I reduced C1R cell line transfected with the HLA class I allele HLA-A*11:01.

2022-02-15 | PXD028985 | Pride

Workingtitle HLA-B*57:01 CBZ EPX impact

Project description:Impact of CBZ and EPX treatment on a with HLA-B*57:01 tranduced cell line compared to the parental cell line

2023-05-10 | PXD037502 | Pride

DDA acquisition of the immunopeptidomes of C1R-B*57:01 and C1R-A*02:01 cell lines

Project description:Immunopeptidomes are the peptide repertoires bound by the molecules encoded by the major histocompatibility complex (MHC) (human leukocyte antigen (HLA) in humans). These HLA-peptide complexes are presented on the cell surface for immune T cell recognition. Immunopeptidomics utilizes tandem mass spectrometry (MS/MS) to identify and quantify peptides bound to HLA molecules. The dataset deposited here contains the DDA runs used to generate the spectral libraries of the HLA-bound peptides purified and isolated from C1R-B*57:01 and C1R-A*02:01 cell lines.

2023-02-13 | PXD034429 | Pride

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